Increased protein kinase C theta in skeletal muscle of diabetic patients
Increased protein kinase C theta in skeletal muscle of diabetic patients
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DOI：
10.1053/meta.2001.22512
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发表时间：
2001-05-01
影响因子：
9.8
通讯作者：
Dohm, GL
中科院分区：
文献类型：
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作者：
Itani, SI;Pories, WJ;Dohm, GL
在这项研究中，我们研究了蛋白激酶C（PKC）蛋白和活性是否增加，在人类糖尿病患者的骨骼肌。使用Western分析，在来自肥胖（高胰岛素血症）的人腹直肌骨骼肌中测量颗粒部分中不同PKC亚型（β、θ、δ、μ和ζ）的蛋白质含量。正常血糖）和肥胖糖尿病（高胰岛素血症，高血糖）受试者中，与非糖尿病对照相比，糖尿病患者肌肉颗粒部分中的PKC θ蛋白含量显著更高。PKC θ的免疫沉淀和其活动进行了测量，在肌肉中的糖尿病和非糖尿病对照。与非糖尿病对照组相比，糖尿病患者肌肉中PKC θ活性显著增加。因此，PKC θ蛋白含量和活性在糖尿病患者肌肉中的颗粒级分中显著增加，表明这种亚型参与糖尿病。PKC α蛋白主要分布在胞浆中。与非糖尿病对照组相比，糖尿病患者肌肉中胞浆PKC θ蛋白含量无变化。因此，颗粒相关PKC θ的增加可能是由于易位和激活，而不是蛋白质质量的增加。Copyright（C）2001 by W.B.桑德斯公司
In this study we have investigated whether protein kinase C (PKC) protein and activity are increased in skeletal muscle of human diabetic patients. The protein content of different PKC isoforms (beta, theta, epsilon, delta, mu, and zeta) in the particulate fraction was measured, using Western analysis, in human rectus abdominus skeletal muscle from obese (hyperinsulinemic. normoglycemic) and obese diabetic (hyperinsulinemic, hyperglycemic) subjects, PKC theta protein content was significantly higher in the particulate fraction of muscle from diabetic patients compared with the nondiabetic controls. PKC theta was immunoprecipitated and its activity was measured in muscle from diabetic and nondiabetic controls. There was a significant increase in PKC theta activity in muscle from diabetic patients compared with muscle from nondiabetic controls. Therefore, both PKC theta protein content and activity were significantly increased in the particulate fraction in muscle from diabetic patients, suggesting the involvement of this isoform in diabetes. Most of the PKC a protein was found in the cytosol. There was no change in cytosolic PKC theta protein content in muscle from diabetic patients compared with muscle from nondiabetic controls. Thus, the increase in particulate-associated PKC theta was likely due to translocation and activation rather than an increase in protein mass. Copyright (C) 2001 by W.B. Saunders Company.