Development of XRM-MacConkey agar selective medium for the isolation of Escherichia albertii
Development of XRM-MacConkey agar selective medium for the isolation of Escherichia albertii
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DOI：
10.1016/j.diagmicrobio.2020.115006
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发表时间：
2020-05-01
影响因子：
2.9
通讯作者：
Yamasaki, Shinji
中科院分区：
文献类型：
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作者：
Hinenoya, Atsushi;Nagano, Keigo;Yamasaki, Shinji
阿尔伯蒂埃希氏菌 (Escherichia albertii) 越来越被认为是一种新兴病原体。然而，艾伯特艾伯球菌选择性培养基的缺乏是临床和流行病学研究的瓶颈。在本研究中，开发并评估了一种名为 XRM-MacConkey 琼脂的阿尔伯蒂埃默氏菌选择性培养基，该培养基是改良的麦康基琼脂，添加了木糖 (X)、鼠李糖 (R) 和蜜二糖 (M)，而不是乳糖。所有 49 个 E. albertii 和 23 个中的 6 个不同物种都生长为无色菌落，而其余 17 个物种则生长为红色菌落。当用加标的健康人粪便进行检查时，该培养基对阿尔伯氏菌的检测限为 10(5) CFU/g 粪便。此外，从 7 个艾伯氏菌阳性腹泻粪便中获得的 XRM-MacConkey 琼脂上的无色菌落始终是艾伯氏菌。相比之下，MacConkey、DHL 和 mEA 琼脂上的无色菌落分别有 57%、18% 和 36% 是非大肠杆菌菌落。阿尔贝蒂。我们得出结论，XRM-MacConkey 琼脂可以专门用于分离艾伯特艾伯氏菌。 (C) 2020 Elsevier Inc. 保留所有权利。
Escherichia albertii has increasingly been recognized as an emerging pathogen. However, lack of selective medium for E. albertii is the bottleneck for clinical and epidemiological investigations. In this study, a selective medium for E. albertii named XRM-MacConkey agar, which is modified MacConkey agar supplemented with xylose (X), rhamnose (R), and melibiose (M) instead of lactose, was developed and evaluated. All 49 E. albertii and 6 different species out of 23 grew as colorless colonies, whereas 17 remaining species grew as red colonies. Detection limit of E. albertii by this medium was 10(5) CFU/g stool when examined with spiked healthy human stool. Further-more, colorless colonies on XRM-MacConkey agar obtained from 7 E. albertii-positive diarrheal stools were consistently E. albertii. In contrast, 57%, 18%, and 36% colorless colonies on MacConkey, DHL, and mEA agars, respectively, were non-E. albertii. We concluded that XRM-MacConkey agar could specifically be used for isolation of E albertii. (C) 2020 Elsevier Inc. All rights reserved.