Inhibition of transcription by immunoglobulins directed against the ribonucleoprotein of homotypic and heterotypic vesicular stomatitis viruses
Inhibition of transcription by immunoglobulins directed against the ribonucleoprotein of homotypic and heterotypic vesicular stomatitis viruses
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针对同型和异型水泡性口炎病毒核糖核蛋白的免疫球蛋白对转录的抑制
DOI：
10.1128/jvi.25.2.675-684.1978
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发表时间：
1978
影响因子：
5.4
通讯作者：
R. Wagner
中科院分区：
文献类型：
--
作者：
A. Carroll;R. Wagner
用从印第安纳州(VS)和新泽西州(VSNJ)血清型水疱性口炎(VS)病毒体获得的仅含RNA和N蛋白(核糖核蛋白，RNP)的纯化核衣壳免疫兔，获得特异性抗血清。通过选择性沉淀不含L蛋白和NS蛋白的同型RNPInd，证实了抗RNPInd血清的特异性，抗RNPInd血清还选择性地沉淀存在于感染细胞胞浆中的可溶性N蛋白，但共沉淀了少量污染的可溶性NS蛋白。从各同型抗血清制备的免疫球蛋白在体外明显抑制VSInd和VSNJ病毒粒子的转录。抗RNPInd和抗RNPNJ免疫球蛋白也通过抑制异型病毒粒子的转录而表现出交叉反应，但程度比同型反应小得多。抗RNPInd免疫球蛋白不抑制抗原性无关的ChandiPura横纹夜蛾病毒的转录，但抗RNPNJ免疫球蛋白对转录的抑制作用非常有限。抗RNPInd免疫球蛋白的转录抑制活性不依赖于RNP免疫沉淀活性，RNP免疫沉淀活性可以在抗转录酶活性丧失之前被稀释。抗RNPind免疫球蛋白似乎通过阻止RNA转录本的延长而不是启动或重新启动来对转录产生影响。
Specific antisera were raised by immunization of rabbits with purified nucleocapsids containing only RNA and N protein (ribonucleoprotein, RNP) obtained from vesicular stomatitis (VS) virions of the Indiana (VSInd) and the New Jersey (VSNJ) serotypes. The specificity of anti-RNPInd serum was demonstrated by selective precipitation of homotypic RNPInd devoid of L and NS proteins; anti-RNPInd serum also selectively precipitated soluble N protein present in cytoplasm of infected cells, but co-precipitated a limited amount of contaminating soluble NS protein. Immunoglobulins prepared from each homotypic antiserum markedly inhibited in vitro transcription of VSInd and VSNJ virions. Anti-RNPInd and anti-RNPNJ immunoglobulins also exhibited cross-reactivity by inhibiting transcription of heterotypic virions, but only to a much lesser degree than in the homotypic reaction. Anti-RNPInd immunoglobulin did not inhibit transcription of the antigenically unrelated Chandipura rhabdovirus, but anti-RNPNJ immunoglobulin did to a very limited extent. The transcription inhibitory activity of anti-RNPInd immunoglobulin was not dependent on RNP immunoprecipitation activity, which could be diluted out well before loss of antitranscriptase activity. Anti-RNPind immunoglobulin appeared to exert its effect on transcription by blocking elongation rather than initiation or reinitiation of RNA transcripts.