Biotransformation of ginsenoside Rb1 to Gyp-XVII and minor ginsenoside Rg3 by endophytic bacterium Flavobacterium sp. GE 32 isolated from Panax ginseng
Biotransformation of ginsenoside Rb1 to Gyp-XVII and minor ginsenoside Rg3 by endophytic bacterium Flavobacterium sp. GE 32 isolated from Panax ginseng
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DOI：
10.1111/lam.13090
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发表时间：
2019-02-01
影响因子：
2.4
通讯作者：
Fu, Y.
中科院分区：
文献类型：
--
作者：
Fu, Y.
稀有的人参皂苷Rg 3因其良好的生理活性和迫切的需求而备受关注。有许多途径可以获得抗肿瘤Rg 3，包括化学和生物学方法。其中，微生物水解法转化原人参二醇型皂苷具有转化效率高、条件温和等优点，是一种发展趋势。为了有效提取人参二醇组皂苷，本研究利用人参内生真菌转化人参皂苷Rg 3，并对其进行了初步研究。其代谢途径为：Rb 1-> Gyp-XVII和Rb 1-> Rd -> Rb 3。16 S rDNA序列分析表明，GE 32菌株属于黄杆菌属（Flavobacterium）。以上结果表明，利用植物内生细菌GE 32生产稀有稀土Rg 3的工艺是可行的，可用于工业化生产和应用。本研究首次报道了可培养的人参内生细菌产β-葡萄糖苷酶。黄杆菌GE 32能将主要的β Rb 1转化为Gyp-XVII和次要的β Rb 3。GE 32菌株具有在制药工业中制备小分子辅酶Rg 3的潜力。
The rare ginsenoside Rg3 is attracting more attention because of its good physiological activity and urgent need. There are many pathways to obtain ginsenoside Rg3, including chemical and biological methods. Among these, the conversion of the protopanaxadiol-type ginsenosides by microbial hydrolysis is a trend due to its high efficiency and mild conditions. For effectively extracting from the other panaxadiol saponins, the conversion process for ginsenoside Rg3 was investigated using beta-glycosidase-producing endophytic fungus in Panax ginseng in this study. The metabolic pathways are as follows: ginsenoside Rb1 -> Gyp-XVII and ginsenoside Rb1 -> ginsenoside Rd -> ginsenoside Rg3. Phylogenetic analysis of 16S rDNA gene sequence, showed that GE 32 strain belonged to Flavobacterium species. These results suggest that the process of rare ginsenoside Rg3 production by endophytic bacteria GE 32 is efficient for the industrial production and application. Significance and Impact of the Study This is the first report on cultivable beta-glycosidase-producing endophytic bacteria from Panax ginseng. Flavobacterium sp. GE32 could convert major ginsenoside Rb1 into Gyp-XVII and minor ginsenoside Rg3. Strain GE 32 has potential to be applied on the preparation for minor ginsenoside Rg3 in pharmaceutical industry.