A Novel Method for Assessing Lamina Cribrosa Structure Ex Vivo Using Anterior Segment Enhanced Depth Imaging Optical Coherence Tomography
A Novel Method for Assessing Lamina Cribrosa Structure Ex Vivo Using Anterior Segment Enhanced Depth Imaging Optical Coherence Tomography
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DOI：
10.1097/ijg.0000000000000685
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发表时间：
2017-07-01
影响因子：
2
通讯作者：
Park, Sung Chul
中科院分区：
文献类型：
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作者：
Chien, Jason L.;Ghassibi, Mark P.;Park, Sung Chul
目的：本研究的目的是探讨眼前段增强深度成像（EDI）光学相干断层扫描（OCT）在离体筛板（LC）成像中的应用。材料和方法：去除前段和玻璃体后，将猪眼的视神经乳头（ONH）组织放置在定制的眼支架上进行成像。使用谱域 OCT 的眼前段模块获得 ONH 的串行 EDI OCT B 扫描（间隔，类似于 35 μm）。测试了各种条件以获得更好质量的 LC 图像。 EDI OCT后，获得连续的组织学切片（切片之间的距离，类似于5μm）。将 OCT 扫描中的 LC 结构与组织学切片中的 LC 结构进行比较。将使用连续 OCT 扫描创建的三维 LC 重建与椎间盘照片中的 LC 结构进行比较。结果：检查了 3 个摘除眼的 ONH。当视网膜和部分层前组织被去除时（质量评分，39.01 +/- 3.30 vs. 26.40 +/- 5.85；P < 0.001）以及当组织在成像过程中保持湿润时（质量评分，38.70 +/- 2.11 vs. 36.18 +/- 5.98；P < 0.001），LC 成像更清晰。固定前后的 LC 图像质量相似（质量评分，38.84 +/- 6.57 vs. 39.21 +/- 9.69；P = 0.79）。 OCT 扫描中识别出的 LC 光束和部分层后神经胶质柱与组织学切片中的相匹配。 3 维重建中的 LC 光束和孔与圆盘照片中的相匹配。结论：通过适当的组织准备，可以在离体眼睛中使用前段 EDI OCT 获得与组织学切片相当的 LC 高分辨率横截面图像。
Purpose: The purpose of this study is to investigate the use of anterior segment enhanced depth imaging (EDI) optical coherence tomography (OCT) for ex vivo lamina cribrosa (LC) imaging.Materials and Methods: After removing anterior segment and vitreous, the optic nerve head (ONH) tissue of porcine eyes was placed on a customized eye holder for imaging. Serial EDI OCT B-scans (interval, similar to 35 mu m) of the ONH were obtained using anterior segment module of spectral-domain OCT. Various conditions were tested for better quality LC images. After EDI OCT, serial histologic sections were obtained (distance between sections, similar to 5 mu m). LC structures in OCT scans were compared with those in histologic sections. Three-dimensional LC reconstructions created using serial OCT scans were compared with LC structures in disc photographs.Results: ONHs of 3 enucleated eyes were examined. The LC was more clearly imaged when the retina and part of the prelaminar tissue were removed (quality score, 39.01 +/- 3.30 vs. 26.40 +/- 5.85; P < 0.001) and when the tissue was kept moist during imaging (quality score, 38.70 +/- 2.11 vs. 36.18 +/- 5.98; P < 0.001). LC image quality was similar before and after fixation (quality score, 38.84 +/- 6.57 vs. 39.21 +/- 9.69; P = 0.79). LC beams and part of retrolaminar glial columns identified in OCT scans matched those in histologic sections. LC beams and pores in 3-dimensional reconstructions matched those in disc photographs.Conclusions: High-resolution cross-sectional images of the LC, comparable to histologic sections, can be obtained using anterior segment EDI OCT in ex vivo eyes with proper tissue preparation.