Clonal proliferation of multipotent stem/progenitor cells in the neonatal and adult salivary glands
Clonal proliferation of multipotent stem/progenitor cells in the neonatal and adult salivary glands
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DOI：
10.1016/j.bbrc.2005.12.031
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发表时间：
2006-02-10
影响因子：
3.1
通讯作者：
Taniguchi, H
中科院分区：
文献类型：
--
作者：
Kishi, T;Takao, T;Taniguchi, H
唾液腺干/祖细胞被认为存在于闰管细胞中，但事实尚不清楚，在本研究中，我们试图澄清干/祖细胞是否存在于下颌下腺中使用集落测定，这是干细胞测定方法之一。采用低密度培养新生大鼠下颌下腺细胞。我们开发了一种新的培养系统，可以促进单细胞集落的形成。集落形成细胞的平均倍增时间为24.7（SD = +/- 7.02）h，表明高增殖效力。当培养基中加入表皮生长因子（EGF）和肝细胞生长因子（HGF）时。克隆集落的数目增加大于没有生长因子培养的那些（13.2 +/-4.18 vs. 4. 5 +/-1.73）。RT-PCR和免疫组化结果显示，在正常涎腺组织中，腺泡、导管和肌上皮细胞系均表达。本研究证实了涎腺干/祖细胞的存在，它们是高度增殖的多能性疾病涎腺细胞。(c)2005年爱思唯尔公司All rights reserved.
Salivary gland stem/progenitor cells are thought to be present in intercalated ductal cells, but the fact is unclear, In this Study, we sought to clarify if stem/progenitor cells are present in submandibular glands using colony assay, which is one of the stem cell assay methods. Using a low-density culture of submandibular gland cells of neonatal rats. we developed,I novel culture system that promotes single cell colony formation. Average doubling time for the colony-forming cells was 24.7 (SD = +/- 7.02) h, indicating high proliferative potency. When epidermal growth factor (EGF) and hepatocyte growth factor (HGF) were added to the medium. the number of clonal colonies increased greater than those cultured without growth factors (13.2 +/- 4.18 vs. 4.5 +/- 1.73). The RT-PCR and immunostaining demonstrated expressing acinar, ductal, and myoepithelial cell lineage markers, This study demonstrated the presence of the salivary gland stem/progenitor cells that are highly proliferative and multipotent ill salivary glands. (c) 2005 Elsevier Inc. All rights reserved.