Bombesin stimulates prolactin and growth hormone release by pituitary cells in culture.
Bombesin stimulates prolactin and growth hormone release by pituitary cells in culture.
复制标题
铃蟾肽刺激培养物中垂体细胞释放催乳素和生长激素。
作者：
J. M. Westendorf;A. Schonbrunn
蛙皮素(BBS)此前已被证明能刺激类固醇刺激的大鼠催乳素和生长激素的分泌。为了确定这些效应是由中枢神经系统介导的还是直接作用于垂体，我们研究了BBS与GH4C1细胞的相互作用。GH4C1细胞是一种合成和分泌PRL和GH的大鼠垂体细胞克隆株。将100 nM BBS作用于GH4C1细胞60min，可使PRL释放增加到对照的140+/-3%(平均值+/-SE)，GH释放增加到对照的133+/-5%。100 nM BBS作用15min内刺激激素分泌，并持续至少2 h，0.5 nM BBS刺激PRL释放半峰值，10 nM多肽刺激作用最大。BBS类似物雷尼托林、列托林和[Tyr4]BBS在100 nM的浓度下，对PRL释放的刺激作用与BBS本身的最大浓度相同。BBS选择性地刺激五种不同克隆的垂体细胞株中的两种释放激素。用1 nM雌二醇和/或100 nM胰岛素处理GH4C1细胞，TRH和BBS对PRL的释放均有更强的刺激作用。当表皮生长因子和血管活性肠肽与BBS同时加入时，PRL的释放比单独使用任何一种多肽时都要大。相反，TRH和BBS的刺激作用不是相加的。生长抑素抑制基础和刺激的催乳素释放。因此，低浓度的BBS可直接刺激体外培养的克隆性垂体细胞株释放PRL和GH。这些结果提示，BBS可能通过直接作用于脑下垂体而刺激体内PRL和GH的分泌。
Bombesin (BBS) has been previously shown to stimulate the secretion of PRL and GH in steroid-primed rats. To determine whether these effects were mediated by the central nervous system or were due to direct action on the pituitary gland, we studied the interaction of BBS with GH4C1 cells, a clonal strain of rat pituitary cells which synthesizes and secretes PRL and GH. The addition of 100 nM BBS to GH4C1 cells for 60 min increased PRL release to 140 +/- 3% of the control value (mean +/- SE) and GH release to 133 +/- 5% of the control value. Stimulation of hormone secretion was observed within 15 min of treatment with 100 nM BBS and continued for at least 2 h. Half-maximal stimulation of PRL release occurred with 0.5 nM BBS, and a maximal effect was observed with 10 nM peptide. The BBS analogs ranatensin, litorin, and [Tyr4]BBS, each at a concentration of 100 nM, caused the same stimulation of PRL release as maximal concentrations of BBS itself. BBS stimulated hormone release selectively in two of five different clonal pituitary cell strains examined. Pretreatment of GH4C1 cells with 1 nM estradiol and/or 100 nM insulin resulted in more powerful stimulation of PRL release by both TRH and BBS. When epidermal growth factor and vasoactive intestinal peptide were added simultaneously with BBS, PRL release was greater than in the presence of either peptide alone. In contrast, the stimulatory effects of TRH and BBS were not additive. Somatostatin inhibited both basal and stimulated PRL release. Thus, low concentrations of BBS can directly stimulate PRL and GH release by a clonal pituitary cell strain in culture. These results suggest that BBS may stimulate PRL and GH secretion in vivo by direct action on the pituitary gland.
胰岛素和17β-雌二醇增加GH4C1细胞的细胞内催乳素含量。
影响因子：
4.8
作者：
Kiino,DR;Dannies,PS
通讯作者：
Dannies,PS