Phosphorus compounds in animal tissues; a comparison of methods for the estimation of nucleic acids.

Phosphorus compounds in animal tissues; a comparison of methods for the estimation of nucleic acids.
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DOI:
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发表时间:
1946-08
期刊:
The Journal of biological chemistry
影响因子:
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通讯作者:
W. C. Schneider
W. C. Schneider
中科院分区:
其他
文献类型:
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作者:
W. C. Schneider

文献摘要

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最近,两种用于估计动物组织中核酸的方法几乎同时出现(3,4)。尽管这些独立进行的实验所采用的技术差别很大,但结果却非常一致。然而,这两种方法所获得的结果显然存在一些差异。由于这个原因,也由于人们对细胞生理学中核酸的研究越来越感兴趣,比较这两种方法在同一组织样品上获得的结果似乎很有意义。本报告描述了用这两种方法分析六种大鼠组织核酸的结果,还描述了一种结合两种方法原理的改进的核酸分析方法。
Recently two methods for the estimation of nucleic acids in animal tissues appeared almost simultaneously (3, 4). Although the techniques employed in these independently conducted experiment,s differed considerably, the results agreed remarkably well. Some discrepancies in the results obtained by the two methods were, however, evident. For this reason and because of the increasing interest in the r of nucleic acids in cellular physiology, it seemed of interest to compare the results obtained by the two methods on the same sample of tissue. The present report describes the resu1t.s of the analysis of six rat tissues for nucleic acids by the two methods and also describes an improved method for nucleic acid analysis which incorporates the principles of both methods.