Droplet Digital PCR and Immunohistochemistry Techniques to Detect Zika Virus in the Central Nervous System of Mice
Droplet Digital PCR and Immunohistochemistry Techniques to Detect Zika Virus in the Central Nervous System of Mice
复制标题
DOI：
10.1007/978-1-0716-0581-3_4
复制
发表时间：
2020-01-01
期刊：
影响因子：
--
通讯作者：
Boivin, Guy
中科院分区：
文献类型：
--
作者：
Enlow, William;Piret, Jocelyne;Boivin, Guy
寨卡病毒（ZIKV）在中枢神经系统（CNS）中的检测是在动物模型中研究感染的发病机制时的关键步骤。病毒载量测定和免疫组织化学（IHC）染色都是定量和定性表征靶组织中病毒感染的有用方法。在这里，我们描述了通过液滴数字PCR进行的病毒RNA载量测定以及通过基于聚合物的IHC进行的蛋白质检测，作为定量和定位小鼠CNS中ZIKV的有效技术。
Detection of Zika virus (ZIKV) in the central nervous system (CNS) is a critical step when studying the pathogenesis of the infection in animal models. Both viral load determination and immunohistochemistry (IHC) staining are useful methods to quantitatively and qualitatively characterize viral infections in target tissues. Here, we describe viral RNA load determination by droplet digital PCR as well as protein detection by polymer-based IHC as effective techniques to quantify and localize ZIKV in the CNS of mice.