GAL4 ACTIVATES TRANSCRIPTION IN DROSOPHILA
GAL4 ACTIVATES TRANSCRIPTION IN DROSOPHILA
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DOI：
10.1038/332853a0
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发表时间：
1988-04-28
期刊：
影响因子：
64.8
通讯作者：
PTASHNE, M
中科院分区：
文献类型：
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作者：
FISCHER, JA;GINIGER, E;PTASHNE, M
GAL4 是一种酵母调节蛋白，它与称为 UASG（半乳糖上游激活序列）的 DNA 序列中的特定位点结合，并激活连锁基因 1-6 的转录。这种激活需要蛋白质的两种功能7,8：位于氨基末端附近的DNA结合域8，以及一个或多个“激活区域”7-11。 “激活区域”呈高酸性9-11（另见参考文献12），并且可以被替换，例如，被设计用于形成带负电荷的两亲性α-螺旋13的短肽。 GAL4 以及带有一个或多个连接到 DNA 结合结构域的“激活区”的缺失衍生物，可在培养的哺乳动物细胞中从连接到 UASG 的哺乳动物启动子激活转录（参考文献 14,15）。在这里，我们表明，当GAL4在果蝇幼虫的特定组织中表达时，刺激与GAL4结合位点连接的果蝇启动子的组织特异性转录。
GAL4 is a yeast regulatory protein that binds to specific sites within a DNA sequence called UASG(galactose upstream activating sequence) and activates transcription of linked genes1–6. This activation requires two functions of the protein7,8: a DNA binding domain located near the amino terminus8, and one or more 'activating regions'7–11. The 'activating regions' are highly acidic9–11(see also ref. 12) and can be replaced, for example, by a short peptide designed to form a negatively charged, amphipathic α-helix13. GAL4, as well as deletion derivatives bearing one or more 'activating regions' attached to the DNA binding domain, activates transcription in cultured mammalian cells from mammalian promoters linked to a UASG(refs 14,15). Here we show that GAL4, when expressed in particular tissues ofDrosophilalarvae, stimulates tissue-specific transcription of aDrosophilapromoter linked to GAL4 binding sites.