Fascin promotes the motility and invasiveness of pancreatic cancer cells
Fascin promotes the motility and invasiveness of pancreatic cancer cells
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Fascin促进胰腺癌细胞的运动和侵袭
DOI：
10.3748/wjg.v17.i40.4470
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发表时间：
2011-10-28
影响因子：
4.3
通讯作者：
Chen, Jie
中科院分区：
文献类型：
--
作者：
Xu, Yan-Feng;Yu, Shuang-Ni;Chen, Jie
目的：探讨肌动蛋白捆绑蛋白（actin- bundle protein，简称fasin）在胰腺癌进展中的作用。方法：将表达人fasin -1的质粒稳定转染到胰腺癌细胞系MIA PaCa-2中。测定了肌动蛋白转染MIA PaCa-2细胞和对照非转染细胞的增殖、细胞周期、运动性、散射性、侵袭性和肌动蛋白丝系统的组织。结果：异质过表达的筋膜蛋白显著增强MIA PaCa-2细胞的运动性、散射性和侵袭性。然而，过表达fastin对MIA PaCa-2细胞增殖和细胞周期的影响很小。此外，肌动蛋白过表达细胞的细胞形态和肌动蛋白丝系统的组织明显改变。当移植到BALB/c-nu小鼠体内时，筋膜蛋白转染的胰腺癌细胞形成实体瘤的速度略慢，但与对照肿瘤相比，这些肿瘤表现出更强的侵袭性。结论：fasin促进胰腺癌细胞的迁移、侵袭和分散，参与胰腺癌细胞的侵袭行为。(C) 2011年白石登。版权所有。
AIM: To explore the role of actin-bundling protein, fascin during the progression of pancreatic cancer.METHODS: The plasmid expressing human fascin-1 was stably transfected into the pancreatic cancer cell line MIA PaCa-2. The proliferation, cell cycle, motility, scattering, invasiveness and organization of the actin filament system in fascin-transfected MIA PaCa-2 cells and control non-transfected cells were determined.RESULTS: Heterogeneous overexpression of fascin markedly enhanced the motility, scattering, and invasiveness of MIA PaCa-2 cells. However, overexpression of fascin had minimal effect on MIA PaCa-2 cell proliferation and cell cycle. In addition, cell morphology and organization of the actin filament system were distinctly altered in fascin overexpressed cells. When transplanted into BALB/c-nu mice, fascin-transfected pancreatic cancer cells developed solid tumors at a slightly slower rate, but these tumors displayed more aggressive behavior in comparison with control tumors.CONCLUSION: Fascin promotes pancreatic cancer cell migration, invasion and scattering, thus contributes to the aggressive behavior of pancreatic cancer cells. (C) 2011 Baishideng. All rights reserved.