How to Build a Complex, Functional Propeller Protein, From Parts.
How to Build a Complex, Functional Propeller Protein, From Parts.
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如何从零件构建复杂的功能性螺旋桨蛋白质。
DOI：
10.1016/j.tibs.2016.02.010
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发表时间：
2016
影响因子：
13.8
通讯作者：
Clark,PatriciaL
中科院分区：
文献类型：
--
作者：
Clark,PatriciaL
通过将祖先序列重建和体外进化相结合，Smodial鉴定了组装成功能性五叶β螺旋桨的单个基序，以及转化为更复杂的现存单链融合体的可能途径。有趣的是，虽然序列多样化使五基序融合不稳定，但它也使易于聚集的中间体不稳定，增加了体内功能蛋白的水平。
By combining ancestral sequence reconstruction andin vitroevolution, Smocket al.identified single motifs that assemble into a functional five-bladed β-propeller, and a likely route for conversion into the more complex, extant single chain fusion. Interestingly, although sequence diversification destabilized five-motif fusions, it also destabilized aggregation-prone intermediates, increasing the level of functional proteinin vivo.