Variation in the ratio of curli and phosphoethanolamine cellulose associated with biofilm architecture and properties
Variation in the ratio of curli and phosphoethanolamine cellulose associated with biofilm architecture and properties
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DOI：
10.1002/bip.23395
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发表时间：
2020-09-07
期刊：
影响因子：
2.9
通讯作者：
Cegelski, Lynette
中科院分区：
文献类型：
--
作者：
Jeffries, Jamie;Thongsomboon, Wiriya;Cegelski, Lynette
细菌生物膜是指由大量细菌聚集在自身产生的细胞外基质（extracellular matrix，ECM）中形成的生物膜，大肠杆菌（Escherichia coli）通过诱导卷曲淀粉样纤维（curli amyloid fibers）和磷酸乙醇胺（phosphoethanolamine，pEtN）纤维素（cellulose）这两种不溶性生物聚合物的组装而形成。强烈的好奇心围绕着细菌如何利用这些淀粉样蛋白-多糖复合物来构建生物膜，以及这些生物聚合物如何使细菌群落受益。定义涉及不溶性聚合物组装体的ECM组合物对分析提出了独特的挑战，并因此对将菌株与定量ECM分子相关物进行比较提出了独特的挑战。在这项工作中，我们提出的结果，从一个总和的部分（13）C固态核磁共振（NMR）分析，以确定卷曲的pEtN纤维素的比例在分离的ECM的E。实验室K12株，AR 3110。我们比较和对比的组成分析和全面的生物膜表型AR 3110和一个良好的研究临床分离，UTI 89。从AR 3110分离的ECM含有相对于卷曲含量的约两倍于UTI 89的量的pEtN纤维素，揭示了菌株之间基质组装原理的可塑性。在三种生物膜模型中研究了两种亲本菌株和一组相关基因突变体，检查：（a）琼脂上的大菌落，（B）液-气界面处的薄膜，和（c）塑料上的生物量积累。我们描述了curli，纤维素，和pEtN修饰对生物膜表型的影响，在这些菌株的直接比较中具有功率。结果表明，curli更强烈地影响粘附力，而pEtN纤维素驱动的凝聚力。它们的个体和组合影响取决于生物膜形态（琼脂、薄膜或塑料相关）和菌株本身。
Bacterial biofilms are communities of bacteria entangled in a self-produced extracellular matrix (ECM).Escherichia colidirect the assembly of two insoluble biopolymers, curli amyloid fibers, and phosphoethanolamine (pEtN) cellulose, to build remarkable biofilm architectures. Intense curiosity surrounds how bacteria harness these amyloid-polysaccharide composites to build biofilms, and how these biopolymers function to benefit bacterial communities. Defining ECM composition involving insoluble polymeric assemblies poses unique challenges to analysis and, thus, to comparing strains with quantitative ECM molecular correlates. In this work, we present results from a sum-of-the-parts(13)C solid-state nuclear magnetic resonance (NMR) analysis to define the curli-to-pEtN cellulose ratio in the isolated ECM of theE. colilaboratory K12 strain, AR3110. We compare and contrast the compositional analysis and comprehensive biofilm phenotypes for AR3110 and a well-studied clinical isolate, UTI89. The ECM isolated from AR3110 contains approximately twice the amount of pEtN cellulose relative to curli content as UTI89, revealing plasticity in matrix assembly principles among strains. The two parent strains and a panel of relevant gene mutants were investigated in three biofilm models, examining: (a) macrocolonies on agar, (b) pellicles at the liquid-air interface, and (c) biomass accumulation on plastic. We describe the influence of curli, cellulose, and the pEtN modification on biofilm phenotypes with power in the direct comparison of these strains. The results suggest that curli more strongly influence adhesion, while pEtN cellulose drives cohesion. Their individual and combined influence depends on both the biofilm modality (agar, pellicle, or plastic-associated) and the strain itself.