Cholesterol determination using protein-templated fluorescent gold nanocluster probes
Cholesterol determination using protein-templated fluorescent gold nanocluster probes
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DOI：
10.1039/c3an01548c
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发表时间：
2013-01-01
期刊：
影响因子：
4.2
通讯作者：
Baker, Gary A.
中科院分区：
文献类型：
--
作者：
Chen, Xi;Baker, Gary A.
我们描述了基于牛血清白蛋白稳定的金纳米簇探针与胆固醇氧化酶（ChOx）共溶解在表面活性剂乳液系统中的可溶性胆固醇荧光生物传感器平台的开发。 ChOx 将胆固醇选择性酶促氧化为 cholest-4-en-3-one，产生化学计量量的 H2O2 副产物，产生猝灭反应，表明胆固醇存在于临床相关水平（LOD 类似于 12 muM）。
We describe the development of a fluorescent biosensor platform for soluble cholesterol based on bovine serum albumin-stabilized gold nanocluster probes co-dissolved with cholesterol oxidase (ChOx) in a surfactant emulsion system. Selective enzymatic oxidation of cholesterol to cholest-4-en-3-one by ChOx produces stoichiometric amounts of H2O2 by-product, generating a quenching response signaling the presence of cholesterol at clinically relevant levels (LOD similar to 12 mu M).