Imaging MALDI Mass Spectrometry of Sphingolipids Using an Oscillating Capillary Nebulizer Matrix Application System
Imaging MALDI Mass Spectrometry of Sphingolipids Using an Oscillating Capillary Nebulizer Matrix Application System
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DOI：
10.1007/978-1-60761-746-4_7
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发表时间：
2010-01-01
期刊：
影响因子：
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通讯作者：
Sullards, M. Cameron
中科院分区：
文献类型：
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作者：
Chen, Yanfeng;Liu, Ying;Sullards, M. Cameron
基质沉积是基质辅助激光解吸/电离质谱 (MALDI-MS) 组织成像的关键步骤。它极大地影响了 MALDI 成像的质量，特别是对于可能容易溶解在基质应用所用溶剂中的分析物（例如脂质）。本章介绍如何使用振荡毛细管雾化器 (OCN) 将基质气溶胶小液滴喷射到样品表面，以改善基质均匀性、减小晶体尺寸并控制溶剂效应。该协议允许通过成像 MALDI-MS 可视化许多不同的脂质种类，特别是 Tay-Sachs/Sandhoff 病组织切片中的鞘脂。这些脂质的结构是通过使用电喷雾电离结合串联质谱（MS/MS 和 MS3）分析组织提取物来鉴定的。这些结果说明了采用 OCN 进行基质沉积的组织成像 MALDI-MS 对于正常生理学和病理学分子分析的有用性。此外，对脑切片中具有不同定位的众多脂质亚类的观察表明，成像 MALDI-MS 可以有效地用于“脂质组学”研究。
Matrix deposition is a critical step in tissue imaging by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). It greatly, affects the quality of MALDI imaging, especially for the analytes (such as lipids) that may easily dissolve in the solvent used for the matrix application. This chapter describes the use of an oscillating capillary nebulizer (OCN) to spray small droplets of matrix aerosol onto the sample surface for improved matrix homogeneity, reduced crystal size, and controlled solvent effects. This protocol allows visualization of many different lipid species and, of particular interest, sphingolipids in tissue slices of Tay-Sachs/Sandhoff disease by imaging MALDI-MS. The structures of these lipids were identified by analysis of tissue extracts using electrospray ionization in conjunction with tandem mass spectrometry (MS/MS and MS3). These results illustrate the usefulness of tissue imaging MALDI-MS with matrix deposition by OCN for the molecular analysis in normal physiology and pathology. In addition, the observation of numerous lipid subclasses with distinct localizations in the brain slices demonstrates that imaging MALDI-MS could be effectively used for "lipidomic" studies.