Impact of iron deficiency anemia on cell-mediated and humoral immunity in children: A case control study.
Impact of iron deficiency anemia on cell-mediated and humoral immunity in children: A case control study.
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DOI：
10.4103/0976-9668.127317
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发表时间：
2014-01
期刊：
影响因子：
--
通讯作者：
Mitra PK
中科院分区：
文献类型：
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作者：
Das I;Saha K;Mukhopadhyay D;Roy S;Raychaudhuri G;Chatterjee M;Mitra PK
铁在免疫调节中的确切作用，特别是在易患缺铁的儿童中，尚不完全清楚。因此，本研究旨在探讨缺铁性贫血(IDA)及其口服补铁治疗对儿童细胞免疫(CMI)和体液免疫(HMI)的影响。符合纳入标准的40名IDA儿童(15岁)和40名年龄匹配的健康儿童参加了这项病例对照研究。用流式细胞仪检测补铁前和补铁后3个月血清免疫球蛋白G(Ig G)、免疫球蛋白A(Ig A)、免疫球蛋白M(Ig M)的变化，并用流式细胞仪检测CD8和CD8淋巴细胞亚群的绝对和相对数量来评价CMI。铁缺乏儿童的CD4+T细胞水平和CD4/CD8比值显著降低(P<；0.05)。补铁可显著提高小鼠外周血中CD4+细胞数和CD4/CD8比值。然而，免疫球蛋白水平在两组之间没有差异。尽管IDA对HMI没有影响，但它显著损害了CMI，在补充铁3个月后，CMI有所改善。
The precise role of iron in immune regulation especially in children vulnerable to iron deficiency is not fully known. Hence, this study was conducted to evaluate the effects of iron deficiency anemia (IDA) and its treatment with oral iron supplementation on cell-mediated immunity (CMI) and humoral immunity (HMI) in children. A total of 40 children (<15 years) with IDA and 40 age-matched healthy children after satisfying the inclusion criteria were enrolled for this case-control study. Flow cytometric evaluation of absolute and relative numbers of cluster of differentiation 4 (CD4) and CD8 (cluster of differentiation 8) lymphocyte subgroups was carried out to assess the CMI and serum Immunoglobulin G (IgG), Immunoglobulin A (IgA), Immunoglobulin M (IgM) were measured to assess the HMI at baseline and 3 months post oral iron supplementation. Significantly lower levels (P < 0.05) of CD4+ T-cells and decreased CD4:CD8 ratios were observed in the iron deficient children. Iron supplementation significantly improved the CD4+ cell counts and CD4:CD8 ratios. However, immunoglobulin levels weren’t different between the two groups. Although IDA did not influence HMI, it significantly impaired CMI, which was improved following iron supplementation for 3 months.