Preparation of poly(N-vinylpyrrolidone-co-pentaerythritol triacrylate) monolithic column for hydrophilic interaction chromatography
Preparation of poly(N-vinylpyrrolidone-co-pentaerythritol triacrylate) monolithic column for hydrophilic interaction chromatography
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DOI：
10.1002/jssc.202201033
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发表时间：
2023-02-21
影响因子：
3.1
通讯作者：
Kang，Jingwu
中科院分区：
文献类型：
--
作者：
Li，Jing;Qiu，Danye;Kang，Jingwu
A method for the preparation of poly(N‐vinylpyrrolidone‐co‐pentaerythritol triacrylate copolymerization)‐based monolithic capillary column was reported for the separation of polar small molecular weight compounds with nano‐liquid chromatography in hydrophilic interaction chromatography mode. The monolithic columns were prepared by in situ copolymerization ofN‐vinylpyrrolidone and a cross‐linker pentaerythritol triacrylate in a binary porogenic agent consisting of methanol and water. The composition of the polymerization solution was systematically optimized in terms of column permeability, theoretical plate number, asymmetric factor, and retention factor. A typical hydrophilic chromatography retention mechanism was observed with a mobile phase composed of a high content of organic solvent. The preparation method is simple and robust, the precursorN‐vinylpyrrolidone is chemically stable, cheap, and easily available. TheN‐vinylpyrrolidone‐based hydrophilic interaction chromatography stationary phase displays satisfactory separation selectivity for a range of polar test analytes, including benzoic acid derivatives, nucleosides, and phenols.
