Human herpesvirus 6A infects human embryonic fibroblasts and induces G2/M arrest and cell death
Human herpesvirus 6A infects human embryonic fibroblasts and induces G2/M arrest and cell death
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人类疱疹病毒 6A 感染人类胚胎成纤维细胞并诱导 G2/M 期停滞和细胞死亡
DOI：
10.1002/jmv.23226
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发表时间：
2012-04-01
影响因子：
12.7
通讯作者：
Yao, Kun
中科院分区：
文献类型：
--
作者：
Li, Lingyun;Gu, Bin;Yao, Kun
人类疱疹病毒6型（Human herpesvirus 6，HHV-6）是一种能够感染不同来源细胞的β-疱疹病毒。在这项研究中，用HHV-6A感染人胚胎成纤维细胞（HEFs）。感染细胞出现明显的细胞病变（CPE）。PCR和免疫组化检测也证实了HEFs对HHV-6A的易感性。研究了HHV-6A感染HEFs的生物学效应。通过[3 H]胸苷掺入和细胞计数测量，感染细胞显示增殖降低。进一步分析表明，HHV-6A感染导致细胞周期停滞在G2/M期，并增加细胞死亡。这是第一次证明HHV-6A感染HEFs会导致细胞特性的深刻改变。医学病毒学杂志84：657663，2012. (C)2011 Wiley Periodicals，Inc.
Human herpesvirus 6 (HHV-6) is a beta-herpesvirus capable of infecting cells from different origin. In this study, infection with HHV-6A of human embryonic fibroblasts (HEFs) was performed. Infected cells showed obvious cytopathic effects (CPE). PCR and immunohistochemical tests also confirmed that HEFs are susceptible to HHV-6A infection. The biological effects of HHV-6A infection on HEFs were studied. Infected cells showed decreased proliferation as measured by [3H] thymidine incorporation and cell counting. Further analysis demonstrated that infection with HHV-6A leads to cell cycle arrest at G2/M phase and increasing cell death. This is the first demonstration that infection of HEFs with HHV-6A causes profound alterations of cell properties. J. Med. Virol. 84:657663, 2012. (C) 2011 Wiley Periodicals, Inc.