Ferredoxin:thioredoxin reductase (FTR) links the regulation of oxygenic photosynthesis to deeply rooted bacteria
Ferredoxin:thioredoxin reductase (FTR) links the regulation of oxygenic photosynthesis to deeply rooted bacteria
复制标题
DOI：
10.1007/s00425-012-1803-y
复制
发表时间：
2013-02-01
期刊：
影响因子：
4.3
通讯作者：
Buchanan, Bob B.
中科院分区：
文献类型：
--
作者：
Balsera, Monica;Uberegui, Estefania;Buchanan, Bob B.
35年前在光合作用的研究中发现，氧化还原调节已经扩展到所有类型的活细胞。我们对这种调节模式的发生、功能和作用机制了解很多，但对其起源和演变却知之甚少。为了帮助填补这一空白，我们利用现有的基因组序列，使追踪系统成员的系统发育根源成为可能，这些成员最初被描述为叶绿体-铁氧还蛋白，铁氧还蛋白：硫氧还蛋白还原酶（FTR），硫氧还蛋白以及靶酶。结果表明：(1)催化亚基FTRc起源于深根的嗜氧微化自养细菌，通过柠檬酸反循环调节CO2固定；(2) FTRc被引入到含氧光合生物中，除了增加一个可变亚基（FTRv）似乎是为了保护Fe-S簇免受氧的侵害外，没有显著的结构变化；(3)从细菌到不同类型的含氧光合生物的进化过程中，系统地添加了新的Trxs和靶酶；(4)在卡尔文-本森循环对二氧化碳固定酶的调控中，氧型调控先于经典的光-暗调控；(5) FTR在氧光合生物中并不普遍存在，在某些早期代表中，其功能似乎被nadp -硫氧还蛋白还原酶所取代；FTRc结构多样化，以满足多种细菌和古菌的生态需求。
Uncovered in studies on photosynthesis 35 years ago, redox regulation has been extended to all types of living cells. We understand a great deal about the occurrence, function, and mechanism of action of this mode of regulation, but we know little about its origin and its evolution. To help fill this gap, we have taken advantage of available genome sequences that make it possible to trace the phylogenetic roots of members of the system that was originally described for chloroplasts-ferredoxin, ferredoxin:thioredoxin reductase (FTR), and thioredoxin as well as target enzymes. The results suggest that: (1) the catalytic subunit, FTRc, originated in deeply rooted microaerophilic, chemoautotrophic bacteria where it appears to function in regulating CO2 fixation by the reverse citric acid cycle; (2) FTRc was incorporated into oxygenic photosynthetic organisms without significant structural change except for addition of a variable subunit (FTRv) seemingly to protect the Fe-S cluster against oxygen; (3) new Trxs and target enzymes were systematically added as evolution proceeded from bacteria through the different types of oxygenic photosynthetic organisms; (4) an oxygenic type of regulation preceded classical light-dark regulation in the regulation of enzymes of CO2 fixation by the Calvin-Benson cycle; (5) FTR is not universally present in oxygenic photosynthetic organisms, and in certain early representatives is seemingly functionally replaced by NADP-thioredoxin reductase; and (6) FTRc underwent structural diversification to meet the ecological needs of a variety of bacteria and archaea.