Tcf7l1 promotes transcription of Kruppel-likefactor 4 during Xenopus embryogenesis.
Tcf7l1 promotes transcription of Kruppel-likefactor 4 during Xenopus embryogenesis.
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Tcf7l1 在非洲爪蟾胚胎发生过程中促进 Kruppel 样因子 4 的转录。
DOI：
10.7555/jbr.32.20170056
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发表时间：
2017-11-01
影响因子：
2.3
通讯作者：
Liu C
中科院分区：
文献类型：
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作者：
Cao Q;Shen Y;Zheng W;Liu H;Liu C
Kruppel样因子4（Klf 4）是一种锌指转录因子，在非洲爪蟾胚胎发育中起重要作用。然而，其在胚胎发生过程中的调控仍不清楚。在这里，我们报告Tcf 7 l1，Wnt信号通路的关键下游转导子，可以促进Klf 4转录和刺激Klf 4启动子活性在早期非洲爪蟾胚胎。此外，放线菌酮处理显示出直接影响Klf 4转录促进Tcf 7 l1。此外，Tcf 7 l1的显性负性形式（dnTcf 7 l1），其缺乏β-catenin结合基序的N-末端，仍然可以激活Klf 4转录，表明这种调节是Wnt/β-catenin独立的。综上所述，我们的研究结果表明，Tcf 7 l1位于Klf 4的上游，以维持其在非洲爪蟾胚胎发生过程中的表达水平。
Kruppel-like factor 4 (Klf4) is a zinc finger transcription factor and plays crucial roles in Xenopus embryogenesis. However, its regulation during embryogenesis is still unclear. Here, we report that Tcf7l1, a key downstream transducer of the Wnt signaling pathway, could promote Klf4 transcription and stimulate Klf4 promoter activity in early Xenopus embryos. Furthermore, cycloheximide treatment showed a direct effect on Klf4 transcription facilitated by Tcf7l1. Moreover, the dominant negative form of Tcf7l1 (dnTcf7l1), which lacks N-terminus of the β-catenin binding motif, could still activate Klf4 transcription, suggesting that this regulation is Wnt/β-catenin independent. Taken together, our results demonstrate that Tcf7l1 lies upstream of Klf4 to maintain its expression level during Xenopus embryogenesis.