Production and characterization of ECM powder: implications for tissue engineering applications
Production and characterization of ECM powder: implications for tissue engineering applications
复制标题
DOI：
10.1016/j.biomaterials.2004.04.042
复制
发表时间：
2005-04-01
期刊：
影响因子：
14
通讯作者：
Badylak, SF
中科院分区：
文献类型：
--
作者：
Gilbert, TW;Stolz, DB;Badylak, SF
研究了两种从猪膀胱中制备颗粒状细胞外基质（ECM）的方法。一种生产粉末的方法涉及快速冷冻冻干形式的材料，然后在研磨机中将其粉碎。第二种方法类似，不同之处在于ECM在快速冷冻之前在NaCl溶液中饱和以在研磨之前在基质内沉淀盐晶体。采用超声筛分、激光衍射、扫描电镜等方法对颗粒的粒度分布和超微结构进行了分析。盐沉淀法产生了一个更均匀的颗粒分布具有较小的平均直径（158与191 μ m）。SEM显示，通过研磨而没有盐沉淀产生的颗粒是不规则形状的片状颗粒。通过盐沉淀法产生的ECM颗粒在外观上是圆形和多孔的，具有许多在1 μ m范围内的颗粒，其倾向于与较大的颗粒以及彼此附聚。我们得出结论，ECM的粉碎形式的生产是可能的，并且颗粒尺寸和形状的均匀性取决于制造方法。(C)2004 Elsevier Ltd.保留所有权利。
Two methods to produce a particulate form of extracellular matrix (ECM) from porcine urinary bladder were investigated. One method to produce the powder involved snap freezing a lyophilized form of the material and then pulverizing it in a grinding mill. The second method was similar except that the ECM was saturated in a solution of NaCl prior to snap freezing to precipitate salt crystals within the matrix before grinding. Several methods were utilized to analyze the particle size distribution and ultrastructure including sonic sifting, laser diffraction, and scanning electron microscopy (SEM). The salt precipitation method yielded a more uniform distribution of particles with a smaller mean diameter (158 vs. 191 mum). SEM showed that the particles produced by grinding without salt precipitation were irregularly shaped, sheet-like particles. ECM particles produced by the salt precipitation method were round and porous in appearance with many particles in the range of I pm which tended to agglomerate with the larger particles and with each other. We conclude that the production of a comminuted form of ECM is possible and that the uniformity of particle size and shape are dependent upon the manufacturing methodology. (C) 2004 Elsevier Ltd. All rights reserved.