Poly(ethylene glycol) hydrogels cross-linked by hydrolyzable polyrotaxane containing hydroxyapatite particles as scaffolds for bone regeneration
Poly(ethylene glycol) hydrogels cross-linked by hydrolyzable polyrotaxane containing hydroxyapatite particles as scaffolds for bone regeneration
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DOI：
10.1163/156856205774576664
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发表时间：
2005-01
期刊：
影响因子：
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通讯作者：
Mariko Fujimoto;M. Isobe;S. Yamaguchi;T. Amagasa;A. Watanabe;T. Ooya;N. Yui
中科院分区：
文献类型：
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作者：
Mariko Fujimoto;M. Isobe;S. Yamaguchi;T. Amagasa;A. Watanabe;T. Ooya;N. Yui
由含有羟基磷灰石颗粒的可水解聚轮烷（PRX-HAp）交联的聚（乙二醇）（PEG）水凝胶被开发为骨再生支架。制备了五种具有不同组成的聚轮烷、PEG和HAp颗粒的支架，以利用大鼠原代培养的成骨细胞体外检查细胞粘附。接种后7天，观察到细胞在具有相同重量比的聚轮烷和HAp颗粒的PRX-HAp上良好附着。这些结果表明HAp颗粒对于细胞粘附和存活是必需的，但是较高比例的颗粒不适合细胞粘附。将大鼠成骨细胞和 PRX-HAp 的复合材料植入同基因大鼠皮下，并在植入后 5 周收获。在组织学分析中，成骨细胞样细胞沿着PRX-HAp的表面排列，并且在成骨细胞样细胞队列和PRX-HAp之间的区域中观察到类骨样组织。这些图像与膜内骨化相似，预计骨再生发生在 PRX-HAp 表面。这项研究强烈表明 PRX-HAp 作为骨再生支架的巨大潜力。
Poly(ethylene glycol) (PEG) hydrogels cross-linked by a hydrolyzable polyrotaxane containing hydroxyapatite particles (PRX-HAp) were developed as scaffolds for bone regeneration. Five scaffolds with various composition of the polyrotaxane, PEG and HAp particles were prepared to examine cell adhesion in vitro using rat primary cultured osteoblast. Cells were observed to attach well on a PRX-HAp that have the same weight ratio of the polyrotaxane and HAp particles at 7 days after seeding. These results indicate that HAp particles are necessary for cell adhesion and survival, but a higher ratio of the particles is not suitable for cell adhesion. The composites of rat osteoblast and the PRX-HAp were implanted subcutaneously in syngeneic rats and harvested at 5 weeks after implantation. In histological analysis, osteoblast-like cells became arrayed along the surface of the PRX-HAp, and osteoid-like tissues were observed in the region between a queue of osteoblast-like cells and PRX-HAp. These images are similar to intramembranous ossification, and it is expected that bone regeneration occurs on the surface of the PRX-HAp. This study strongly suggests the great potential of the PRX-HAp as scaffolds for bone regeneration.