An electrochemical DNA-sensor developed with the use of methylene blue as a redox indicator for the detection of DNA damage induced by endocrine-disrupting compounds
An electrochemical DNA-sensor developed with the use of methylene blue as a redox indicator for the detection of DNA damage induced by endocrine-disrupting compounds
复制标题
使用亚甲蓝作为氧化还原指示剂开发的电化学 DNA 传感器，用于检测内分泌干扰化合物引起的 DNA 损伤
DOI：
10.1016/j.aca.2015.02.050
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发表时间：
2015-03-31
影响因子：
6.2
通讯作者：
Kokot, Serge
中科院分区：
文献类型：
--
作者：
Lin, Xiaoyun;Ni, Yongnian;Kokot, Serge
研制了一种能间接检测双酚A（BPA）、4-壬基酚（NP）和4-叔辛基酚（OP）三种内分泌干扰物（EDCs）引起的DNA损伤的电化学生物传感器。使用亚甲蓝（MB）染料作为氧化还原指示剂。将dsDNA/氧化石墨烯-壳聚糖/金纳米粒子组装到玻碳电极（GCE）上，制备了dsDNA/GO-CS/AuNPs/GCE传感器。采用电化学阻抗谱（EIS）、循环伏安法（CV）和扫描电镜（SEM）等技术对其进行了表征。研究了双链DNA/GO-CS/AuNPs膜对MB染料的负载/释放过程，结果表明该过程是可逆的。在此基础上，该传感器用于测量膜中完整和受损dsDNA的负载能力之间的差异。该传感器成功地应用于电化学检测DNA损伤。在BPA、NP和OP化合物存在下，DNA损伤前后MB的微分脉冲伏安（DPV）峰电流比线性增加，处理范围为10-60 min，损伤率分别为0.0069、0.0044和0.0031 min（-1）。这些结果通过结合常数证实：2.09 × 10（6）M（-1）（BPA-DNA）、1.28 × 10（6）M（-1）（NP-DNA）和9.33 × 10（5）M（-1）（OP-DNA），所有这些都是使用差分脉冲溶出伏安法（DPSV）获得的。(c)2015爱思唯尔B. V.保留所有权利。
An electrochemical biosensor capable of indirect detection of DNA damage induced by any one of the three endocrine-disrupting compounds (EDCs) - bisphenol A (BPA), 4-nonylphenol (NP) and 4-t-octylphenol (OP), has been researched and developed. The methylene blue (MB) dye was used as the redox indicator. The glassy carbon electrode (GCE) was modified by the assembled dsDNA/graphene oxide-chitosan/gold nano-particles to produce a dsDNA/GO-CS/AuNPs/GCE sensor. It was characterized with the use of electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and scanning electron microscopy (SEM) techniques. The loading/release of the MB dye by the dsDNA/GO-CS/AuNPs film was investigated, and the results showed that the process was reversible. Based on this, the sensor was used to measure the difference between the loading capabilities of intact and damaged dsDNA in the films. The sensor was then successfully applied to detect DNA damage electrochemically. The differential pulse voltammetry (DPV) peak current ratio for MB, observed before and after DNA damage, increased linearly in the presence the BPA, NP or OP compounds; the treatment range was 10-60 min, and the respective damage rates were 0.0069, 0.0044 and 0.0031 min(-1), respectively. These results were confirmed by the binding constants: 2.09 x 10(6)M(-1) (BPA-DNA), 1.28 x 10(6)M(-1) (NP-DNA) and 9.33 x 10(5)M(-1) (OP-DNA), all of which were obtained with the use of differential pulse stripping voltammetry (DPSV). (c) 2015 Elsevier B.V. All rights reserved.