CELLULAR REPOPULATION OF DEEP-FROZEN MENISCAL AUTOGRAFTS - AN EXPERIMENTAL-STUDY IN THE DOG
CELLULAR REPOPULATION OF DEEP-FROZEN MENISCAL AUTOGRAFTS - AN EXPERIMENTAL-STUDY IN THE DOG
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DOI：
10.1016/0749-8063(92)90003-t
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发表时间：
1992-12-01
期刊：
影响因子：
4.7
通讯作者：
WARREN, RF
中科院分区：
文献类型：
--
作者：
ARNOCZKY, SP;DICARLO, EF;WARREN, RF
本研究评价了深冻自体髂骨移植物的细胞再增殖。切除成年狗的内侧胫骨，在液氮（-196 ℃）中深冻10分钟，并原位重新植入关节中。发现深度冷冻有效地杀死半月板内的所有细胞，如通过不存在（Na 2SO 4）-S-35掺入所确定的。在膝关节内原位置换后，滑膜细胞重新增殖，这些细胞似乎来源于邻近的滑膜。这些细胞迁移到半月板表面，并开始侵入组织的深层。然而，即使在6个月后，半月板的中央核心仍然是无细胞的。虽然新的细胞似乎调节成外观类似于椎间盘纤维软骨细胞的细胞，但这些新分化细胞的确切表型表达尚未确定。组织学改变，表现为半月板浅层胶原结构的正常方向丧失，在6个月时明显，表明重塑现象可能与细胞再生相关。虽然尚未对这些标本进行生物材料研究，但在胶原蛋白方向上观察到的形态学变化表明，重新填充的半月板的材料特性可能发生变化。本研究的临床意义在于，与深冻异体半月板移植物的细胞再增殖相关的结构重塑可能使移植的半月板更容易损伤。
This study evaluated the cellular repopulation of deep-frozen meniscal autografts. Medial menisci of adult dogs were excised, deep-frozen in liquid nitrogen (- 196-degrees-C) for 10 min, and orthotopically reimplanted into the joint. Deep-freezing was found to effectively kill all the cells within the meniscus as determined by the absence of (Na2SO4)-S-35 incorporation. Following orthotopic replacement within the knee joint, menisci were repopulated with cells that seemed to originate from the adjacent synovium. These cells migrated over the surface of the meniscus and began to invade the deeper layers of the tissue. However, even after 6 months, the central core of the meniscus remained acellular. While the new cells appeared to modulate into cells that are similar in appearance to meniscal fibrochondrocytes, the exact phenotypic expression of these newly differentiated cells has yet to be determined. Histological alterations, as manifested by a loss of normal orientation of the collagen architecture of the superficial layers of the meniscus, was evident at 6 months and suggests that a remodeling phenomenon may be associated with the cellular repopulation. While biomaterial studies have not been carried out on these specimens, the morphologic alterations observed in the collagen orientation would suggest a possible alteration in the material properties of the repopulated meniscus. The clinical implication of this study is that the structural remodeling associated with the cellular repopulation of deep-frozen meniscal allografts may make the transplanted meniscus more susceptible to injury.