Protein storage and reproduction increase in grasshoppers on a diet matched to the amino acids of egg yolk protein
Protein storage and reproduction increase in grasshoppers on a diet matched to the amino acids of egg yolk protein
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DOI：
10.1242/jeb.244450
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发表时间：
2022-09-01
影响因子：
2.8
通讯作者：
Hahn, Daniel A.
中科院分区：
文献类型：
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作者：
Hatle, John D.;Maslikova, Victoriya;Hahn, Daniel A.
动物的饮食对支持发育至关重要，蛋白质是关键。储存的营养物质的积累可以支持发育事件，如蜕皮和繁殖的开始。农业研究已经解决了膳食蛋白质质量如何影响生长，但很少有研究涉及膳食蛋白质质量对发育过渡的影响。研究如何饮食质量可能会影响蛋白质的储存和发展是可能的节肢动物，储存蛋白质的血淋巴。我们假设，与蛋黄蛋白（卵黄蛋白原，Vg）的前体相匹配的氨基酸组成的饮食将是高品质的，并支持鸡蛋生产和储存蛋白的积累。蚱蜢每天喂食两种等氮氨基酸溶液之一：Vg平衡（与Vg匹配）或不平衡（氨基酸总摩尔数相同，但与蛋黄不匹配）。我们测量了繁殖和储存蛋白质水平在一系列血淋巴样品从个人。Vg平衡组比不平衡组有更大的繁殖和更大的储存蛋白累积水平。即使喂给Vg平衡组的氨基酸与不平衡组的氨基酸相比，与贮藏蛋白的匹配性并不好，也会发生这种情况。此外，产卵时间是最好的解释相结合的饮食，年龄在最高水平的存储蛋白六聚体-270和积累的六聚体-90。我们的研究加强了储存蛋白质和生殖承诺之间的联系，并表明膳食蛋白质质量对蛋白质储存和生殖至关重要。
The diets of animals are essential to support development, and protein is key. Accumulation of stored nutrients can support developmental events such as molting and initiation of reproduction. Agricultural studies have addressed how dietary protein quality affects growth, but few studies have addressed the effects of dietary protein quality on developmental transitions. Studies on how dietary quality may affect protein storage and development are possible in arthropods, which store proteins in the hemolymph. We hypothesized that diets with a composition of amino acids that matches the precursor of egg yolk protein (vitellogenin, Vg) will be high quality and support both egg production and accumulation of storage proteins. Grasshoppers were fed one of two isonitrogenous solutions of amino acids daily: Vg-balanced (matched to Vg) or Unbalanced (same total moles of amino acids, but not matched to egg yolk). We measured reproduction and storage protein levels in serial hemolymph samples from individuals. The Vg-balanced group had greater reproduction and greater cumulative levels of storage proteins than did the Unbalanced group. This occurred even though amino acids fed to the Vg-balanced group were not a better match to storage protein than were the amino acids fed to the Unbalanced group. Further, oviposition timing was best explained by a combination of diet, age at the maximum level of storage protein hexamerin-270 and accumulation of hexamerin-90. Our study tightens the link between storage proteins and commitment to reproduction, and shows that dietary protein quality is vital for protein storage and reproduction.