Formation of self-assembled glycolipid nanotubes with bilayer sheets.
Formation of self-assembled glycolipid nanotubes with bilayer sheets.
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具有双层片的自组装糖脂纳米管的形成。
DOI：
10.1166/jnn.2007.406
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发表时间：
2007
影响因子：
--
通讯作者：
S. Isoda
中科院分区：
文献类型：
--
作者：
Kaname Yoshida;H. Minamikawa;S. Kamiya;T. Shimizu;S. Isoda
采用低温透射电子显微镜结合快速冷冻制样技术，对自组装糖脂纳米管(LNT)在水中的卷曲形态进行了细致的研究。LNTs是在一系列自组装条件下获得的：糖脂N-(11-cis-octadecenoyl)-beta-D-glucopyranosylamine，水分散体煮沸，然后逐渐冷却，并在室温下孵育数天。低温电子显微镜图像显示，LNT壁为多层结构，层间距约为4.7 nm。这些层对应于组成脂双分子层。从精确的低温电子显微镜观察和分析的结果，我们证实了完整的自组装糖脂纳米管中的脂类双层膜的卷曲形态。
Rolled-up morphology of bilayer sheets in a self-assembled glycolipid nanotube (LNT) in water was carefully examined by using a cryogenic transmission electron microscope (cryo-TEM) with a rapid-freezing specimen-preparation technique. The LNTs were obtained under a series of self-assembly conditions: boiling of an aqueous dispersion of glycolipid N-(11-cis-octadecenoyl)-beta-D-glucopyranosylamine, subsequent gradual cooling, and incubation at room temperature for several days. Cryo-TEM images revealed that the LNT walls consist of a multilayer structure with interlayer distance of about 4.7 nm. These layers correspond to constituent lipid bilayers. From the result of precise cryo-TEM observations and analyses, we confirmed the rolled-up morphology of the lipid bilayer sheets in a complete self-assembled glycolipid nanotube.