Effects of photodeoxygenation on cell biology using dibenzothiophene S-oxide derivatives as O(3P)-precursors
Effects of photodeoxygenation on cell biology using dibenzothiophene S-oxide derivatives as O(3P)-precursors
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DOI：
10.1007/s43630-021-00136-5
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发表时间：
2021-11-25
影响因子：
3.1
通讯作者：
McCulla, Ryan D.
中科院分区：
文献类型：
--
作者：
Isor, Ankita;O'Dea, Austin T.;McCulla, Ryan D.
二苯并噻吩氧化物S及其衍生物的光脱氧作用被用来产生原子氧[O(P-3)]，以考察其对蛋白质、核酸和脂类的影响。O(P-3)独特的反应性和选择性在生物分子和基于细胞的研究中显示出不同的氧化产物和结果。为了了解其对细胞的全球影响的范围，我们用2，8-二乙酰氧甲基二苯并噻吩氧化物和UV-A光处理MDAMB-231细胞，使其产生O(P-3)，而不针对特定的细胞细胞器。用细胞存活率和细胞周期时相测定分析细胞对O(P-3)释放的反应。当细胞受到较高浓度的亚砜和UV-A光处理时，细胞死亡。然而，亚砜的UV-A辐射对细胞周期时相的影响并不显著。我们进一步进行了RNA-Seq分析，以研究潜在的生物学过程，虽然紫外线照射本身影响了基因的表达，但有9个上调和8个下调的基因可以归因于光去氧。
Photodeoxygenation of dibenzothiophene S-oxide and its derivatives have been used to generate atomic oxygen [O(P-3)] to examine its effect on proteins, nucleic acids, and lipids. The unique reactivity and selectivity of O(P-3) have shown distinct oxidation products and outcomes in biomolecules and cell-based studies. To understand the scope of its global impact on the cell, we treated MDA-MB-231 cells with 2,8-diacetoxymethyldibenzothiophene S-oxide and UV-A light to produce O(P-3) without targeting a specific cell organelle. Cellular responses to O(P-3)-release were analyzed using cell viability and cell cycle phase determination assays. Cell death was observed when cells were treated with higher concentrations of sulfoxides and UV-A light. However, significant differences in cell cycle phases due to UV-A irradiation of the sulfoxide were not observed. We further performed RNA-Seq analysis to study the underlying biological processes at play, and while UV-irradiation itself influenced gene expression, there were 9 upregulated and 8 downregulated genes that could be attributed to photodeoxygenation.[GRAPHICS]