Microsatellite instability at multiple loci in gastric carcinoma: Clinicopathologic implications and prognosis
Microsatellite instability at multiple loci in gastric carcinoma: Clinicopathologic implications and prognosis
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DOI：
10.1053/gast.1996.v110.pm8536886
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发表时间：
1996-01-01
期刊：
影响因子：
29.4
通讯作者：
SobrinhoSimoes, M
中科院分区：
文献类型：
--
作者：
DosSantos, NR;Seruca, R;SobrinhoSimoes, M
背景和目标：微卫星不稳定性复制错误（RER）阳性表型是胃癌中常见的遗传变异，RER阳性与阴性胃癌的临床关系尚不明确，本研究旨在探讨微卫星位点改变数目与胃癌临床病理特征的关系。应用聚合酶链反应（PCR）技术，对61例胃癌组织中的5个或6个微卫星位点进行了分析。结果如下：21例癌（34.4%）有微卫星不稳定性：1个位点7例，2个位点2例，多个位点12例（无、1或2个和2个以上的RER阳性基因座）显示，RER阴性癌和具有1或2个RER阳性基因座的癌共享与具有多个RER阳性基因座的癌不同的特征，后者均为肠型或非典型亚型，与其他两组相比，前者DNA含量较低，淋巴样浸润较明显，淋巴结转移较少。结论：从临床角度来看，在单个或少数位点发现微卫星不稳定并不能使一个病例成为突变表型，多个RER阳性位点的胃癌具有特殊的临床病理特征，预后较好。
Background & Aims: Microsatellite instability (replication error [RER]-positive phenotype) is a frequent genetic alteration in gastric carcinomas, The clinical relationship between RER-positive and RER-negative gastric tumors is poorly characterized, The aim of this study was to investigate the relationship between the number of altered microsatellite loci and the clinicopathologic features of gastric carcinoma, Methods: Five or 6 microsatellite loci were analyzed in 61 gastric carcinomas using polymerase chain reaction. Results: Twenty-one carcinomas (34.4%) had microsatellite instability: 7 at 1 locus, 2 at 2 loci, and 12 at multiple loci, The comparison between the three groups (with none, 1 or 2, and more than 2 RER-positive loci) showed that RER-negative carcinomas and carcinomas with 1 or 2 RER-positive loci share features that differ from those of carcinomas with multiple RER-positive loci, The latter were all of the intestinal or atypical subtype acid had lower DNA content, more prominent lymphoid infiltration, and less prevalent nodal metastases than carcinomas in the other two groups. The patients with carcinomas showing multiple RER-positive loci had a better prognosis, Conclusions: The finding of microsatellite instability in a single or few loci does not qualify a case as a mutator phenotype from a clinical standpoint, Gastric tumors with multiple RER-positive loci have a particular clinicopathologic profile leading to a better outcome.