Quantification of collagen organization and extracellular matrix factors within the healing ligament.
Quantification of collagen organization and extracellular matrix factors within the healing ligament.
复制标题
DOI：
10.1017/s1431927611011925
复制
发表时间：
2011-10
影响因子：
2.8
通讯作者：
Vanderby, Ray
中科院分区：
文献类型：
--
作者：
Chamberlain, Connie S.;Crowley, Erin M.;Kobayashi, Hirohito;Eliceiri, Kevin W.;Vanderby, Ray
III级损伤（即完全撕裂）的韧带愈合涉及形成新韧带的多方面事件链，新韧带的特征比天然组织更像疤痕。重塑过程可能持续数月甚至数年，受伤的韧带从未完全恢复损伤前的机械性能。随着组织工程和再生医学的发展，了解韧带的正常愈合过程并对其进行量化，为创造和评估创新治疗提供了基础。韧带成纤维细胞产生许多ECM组分，包括I型和III型胶原蛋白、核心蛋白聚糖和纤调蛋白。使用先进的组织学，分子生物学和非线性光学成像方法的组合，韧带愈合过程中的早期ECM事件已被更好地表征和定义。首先，显示了损伤后ECM因子的动态变化。其次，确定了与爬行替代相关的因素。最后，开发并使用了一种量化胶原组织的方法。本文所述的每个ECM因子以及纤维组织的时间定量有助于阐明韧带愈合的复杂性。
Ligament healing of a grade III injury (i.e. a complete tear) involves a multifaceted chain of events that forms a neoligament, which is more scar-like in character than the native tissue. The remodeling process may last months or even years with the injured ligament never fully recovering pre-injury mechanical properties. With tissue engineering and regenerative medicine, understanding the normal healing process in ligament and quantifying it provide a basis to create and assess innovative treatments. Ligament fibroblasts produce a number of ECM components, including collagen types I and III, decorin and fibromodulin. Using a combination of advanced histology, molecular biology and nonlinear optical imaging approaches, the early ECM events during ligament healing have been better characterized and defined. First, the dynamic changes in ECM factors after injury are shown. Second, the factors associated with creeping substitution are identified. Finally, a method to quantify collagen organization is developed and used. Each ECM factor described herein as well as the temporal quantification of fiber organization helps elucidate the complexity of ligament healing.