EFFECT OF CILIOSTATIC FACTORS FROM PSEUDOMONAS-AERUGINOSA ON RABBIT RESPIRATORY CILIA
EFFECT OF CILIOSTATIC FACTORS FROM PSEUDOMONAS-AERUGINOSA ON RABBIT RESPIRATORY CILIA
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DOI：
10.1128/iai.51.1.254-262.1986
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发表时间：
1986-01-01
影响因子：
3.1
通讯作者：
SHRYOCK, T
中科院分区：
文献类型：
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作者：
HINGLEY, ST;HASTIE, AT;SHRYOCK, T
铜绿假单胞菌培养上清释放的热稳定因子抑制兔气管上皮功能性纤毛。氯仿萃取从固定相培养物上清液中除去热稳定因子。提取物中至少含有7种可通过薄层色谱法（TLC）分离的组分。纤毛抑制组分被鉴定为吩嗪衍生物、pyo化合物（2-烷基-4-羟基喹啉）和鼠李糖脂（也称为溶血素）。荧光光谱、吸收光谱、薄层色谱相对迁移率、染色特征、气相色谱等是鉴别的依据。每种活性成分的抑制浓度通过运动百分比和搏动频率的定量测量来确定。电镜观察睫状体超微结构的相应损伤。脓化合物在50 μ g/ml的浓度下产生纤毛抑制，但没有明显的超微结构损伤。吩嗪衍生物也抑制纤毛运动并引起一些膜破坏，尽管在400 μ g/ml的实质上更高的浓度下。气管外植体有限暴露于鼠李糖脂导致纤毛停滞，这与纤毛膜改变有关。更广泛地暴露于鼠李糖脂与动力蛋白臂从轴丝的去除。在我们的条件下，浓度为0.5 mg/ml的绿脓菌素不抑制纤毛跳动。数据表明，pyo化合物是铜绿假单胞菌释放的最有效的单位重量纤毛抑制因子，而鼠李糖脂是纤毛结构下最具破坏性的。通过干扰正常纤毛功能，这些纤毛抑制因子可能使铜绿假单胞菌更容易在呼吸道定植。
Heat-stable factors released by Pseudomonas aeruginosa in culture supernatants inhibit functional cilia of rabbit tracheal epithelium. Chloroform extraction removed heat-stable factors from stationary-phase culture supernatants. The extracts contained at least seven components separable by thin-layer chromatography (TLC). Cilioinhibitory components were identified as a phenazine derivative, pyo compounds (2-alkyl-4-hydroxyquinolines), and a rhamnolipid, also known as a hemolysin. Fluorescence and absorption spectra, relative migration on TLC, staining characteristics, and gas chromatography were the basis for identification. Inhibitory concentrations of each active component were established by quantitative measures of percent motility and beat frequency. Corresponding damage to ciliary ultrastructure was examined by electron microscopy. The pyocompounds produced ciliostasis at concentrations of 50 .mu.g/ml, but without obvious ultrastructural lesions. The phenazine derivative also inhibited ciliary motility and caused some membrane disruption, although at substantially greater concentrations of 400 .mu.g/ml. Limited exposure of tracheal explants to the rhamnolipid resulted in ciliostasis which was associated with altered ciliary membranes. More extensive exposure to rhamnolipid was associated with removal of dynein arms from axonemes. Pyocyanin at a concentration of 0.5 mg/ml did not inhibit ciliary beating under our conditions. The data suggest that the pyo compounds are the most effective per weight ciliostatic factors released by P. aeruginosa and rhamnolipids is the most destructive of cilia untrastructure. By interfering with normal ciliary function, these ciliostatic factors may enable P. aeruginosa to more easily colonize the respiratory tract.