Intronic sequences flanking alternatively spliced exons are conserved between human and mouse
Intronic sequences flanking alternatively spliced exons are conserved between human and mouse
复制标题
DOI：
10.1101/gr.1208803
复制
发表时间：
2003-07-01
期刊：
影响因子：
7
通讯作者：
Ast, G
中科院分区：
文献类型：
--
作者：
Sorek, R;Ast, G
对老鼠和人类基因组的序列进行比较，发现了数量惊人的非外显子、非表达的保守序列，这些序列没有任何功能可分配。为了研究这些保守的内含子序列与选择性剪接调控之间的可能相关性，我们开发了一种方法来识别在人类和小鼠中交替剪接的外显子。我们编辑了两个外显子集：一个是交替剪接的保守外显子，另一个是结构性剪接的保守外显子。我们发现，77%的保守选择性剪接外显子两侧都有较长的保守内含子序列。相比之下，只有17%的保守组成剪接外显子的侧翼有这种保守的内含子序列。保守内含子序列的平均长度上游内含子为103个碱基，下游内含子为94个碱基。在紧邻的内含子序列中，上游和下游内含子的平均同源性分别为88%和80%，高于启动子区域77%的保守水平。我们的结果表明，许多在人和鼠之间保守的内含子序列块的功能是调节选择性剪接。
Comparison of the sequences of mouse and human genomes revealed a surprising number of nonexonic, nonexpressed conserved sequences, for which no function could be assigned. To study the possible correlation between these conserved intronic sequences and alternative splicing regulation, we developed a method to identify exons that are alternatively spliced in both human and mouse. We compiled two exon sets: one of alternatively spliced conserved exons and another of constitutively spliced conserved exons. We found that 77% of the conserved alternatively spliced exons were flanked on both sides by long conserved intronic sequences. In comparison, only 17% of the conserved constitutively spliced exons were flanked by such conserved intronic sequences. The average length of the conserved intronic sequences was 103 bases in the upstream intron and 94 bases in the downstream intron. The average identity levels in the immediately flanking intronic sequences were 88% and 80% for the upstream and downstream introns, respectively, higher than the conservation levels of 77% that were measured in promoter regions. Our results suggest that the function of many of the intronic sequence blocks that are conserved between human and mouse is the regulation of alternative splicing.