A physical map around the WAGR complex on the short arm of chromosome 11.
A physical map around the WAGR complex on the short arm of chromosome 11.
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11 号染色体短臂上 WAGR 复合体周围的物理图。
DOI：
10.1016/0888-7543(89)90084-0
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发表时间：
1989
期刊：
影响因子：
4.4
通讯作者：
Bruns,GA
中科院分区：
文献类型：
--
作者：
Gessler,M;Bruns,GA
已使用脉冲场凝胶电泳和一些不常切割的限制性内切酶构建了11号染色体短臂部分（包括WAGR区域）的长程限制性内切酶图谱。总共15.4 Mbp已被详细绘制，从近端11p14延伸到11p12的远端部分。该地图定位了35种不同的DNA探针，并揭示了至少9个具有HTF岛特征的区域，其中一些区域可能是WAGR综合征表型相关的不同基因座的候选区域。该图谱将进一步允许筛选来自具有WAGR相关表型的个体和来自Wilms肿瘤的相关染色体重排的DNA。
A long-range restriction map of part of the short arm of chromosome 11 including the WAGR region has been constructed using pulsed-field gel electrophoresis and a number of infrequently cutting restriction enzymes. A total of 15.4 Mbp has been mapped in detail, extending from proximal 11p14 to the distal part of 11p12. The map localizes 35 different DNA probes and reveals at least nine areas with features characteristic of HTF islands, some of which may be candidates for the different loci underlying the phenotype of the WAGR syndrome. This map will furthermore allow screening of DNA from individuals with WAGR-related phenotypes and from Wilms tumors for associated chromosomal rearrangements.