CLONING AND STRUCTURE OF CDNA-ENCODING ALPHA-LATROTOXIN FROM BLACK-WIDOW SPIDER VENOM
CLONING AND STRUCTURE OF CDNA-ENCODING ALPHA-LATROTOXIN FROM BLACK-WIDOW SPIDER VENOM
复制标题
DOI：
10.1016/0014-5793(90)81250-r
复制
发表时间：
1990-09-17
期刊：
影响因子：
3.5
通讯作者：
GRISHIN, EV
中科院分区：
文献类型：
--
作者：
KIYATKIN, NI;DULUBOVA, IE;GRISHIN, EV
从蜘蛛毒腺cDNA文库中分离编码推定的α-latrotoxin前体的cDNA并测序。该cDNA含有4203个碱基对的开放阅读框架，对应于由1401个氨基酸组成的156 855-Da蛋白质。对推导的一级结构的计算机分析显示，主要在其中心和C末端区域存在各种内部不完美重复。
cDNA encoding the putative α‐latrotoxin precursor was isolated from spider venom glands cDNA library and sequenced. The cDNA contained the 4203 base‐pair open reading frame corresponding to the 156 855‐Da protein composed of 1401 amino acids. Computer analysis of the deduced primary structure revealed the presence of various internal imperfect repeats mainly in its central and C‐tenninal regions.