cDNA cloning and tissue distribution of mRNAs for two proteins that are related to the band 3 Cl-/HCO3- exchanger.
cDNA cloning and tissue distribution of mRNAs for two proteins that are related to the band 3 Cl-/HCO3- exchanger.
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DOI：
10.1016/s0021-9258(19)40253-6
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发表时间：
1990-01
期刊：
影响因子：
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通讯作者：
K. Kudrycki;P. R. Newman;G. Shull
中科院分区：
文献类型：
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作者：
K. Kudrycki;P. R. Newman;G. Shull
已从大鼠胃、脑和肾文库中分离出编码与带3 Cl-/HCO 3-交换器相关的两种蛋白质的互补DNA，命名为B3 RP 2和B3 RP 3。B3 RP 2的长度为1234个氨基酸，并且具有136，644的Mr。B3 RP 3的长度为1227个氨基酸，并且具有135，405的Mr。B3 RP 2和B3 RP 3分别与条带3具有52%和50%的氨基酸同一性，并且彼此具有56%的同一性。B3 RP 2和B3 RP 3的N-末端胞质区域跨越约700个氨基酸，比带3的更广泛。这三种蛋白质的C-末端疏水区表现出高度的氨基酸同一性（64-69%），并且具有非常相似的亲水性特征，表明它们具有相同的跨膜组织。通过北方印迹杂交，使用来自广泛肌肉和非肌肉组织以及胃肠道切片的poly（A）+RNA，检查编码B3 RP 2、B3 RP 3和带3 Cl-/HCO 3-交换器的mRNA的组织分布。B3 RP 2 mRNA在检查的所有组织中表达。最高水平，其中包括至少三种不同的转录本，发生在胃。B3 RP 3 mRNA也由几种不同的转录物组成，具有更有限的组织分布。最高水平出现在心脏，在胃肠道部分，最高水平出现在前胃。在许多组织中观察到条带3 mRNA，但仅在脾和肾中出现高水平表达。检测到5个带3转录本，大小从3.6到4.9个酶，包括3个在心脏中表达的转录本。
Complementary DNAs encoding two proteins that are related to the Band 3 Cl-/HCO3- exchanger, designated B3RP2 and B3RP3, have been isolated from rat stomach, brain, and kidney libraries. B3RP2 is 1234 amino acids in length and has an Mr of 136,644. B3RP3 is 1227 amino acids in length and has an Mr of 135,405. B3RP2 and B3RP3 exhibit 52 and 50% amino acid identity to Band 3, respectively, and 56% identity to each other. The N-terminal cytoplasmic regions of B3RP2 and B3RP3, which span about 700 amino acids, are more extensive than that of Band 3. The C-terminal hydrophobic regions of the three proteins exhibit a high degree of amino acid identity (64-69%) and have very similar hydropathy profiles, suggesting that they have the same transmembrane organization. The tissue distribution of mRNAs encoding B3RP2, B3RP3, and the Band 3 Cl-/HCO3- exchanger were examined by Northern blot hybridization using poly(A)+ RNAs from a broad range of muscle and non-muscle tissues and from sections of the gastrointestinal tract. B3RP2 mRNAs are expressed in all tissues examined. The highest levels, which include at least three different transcripts, occur in stomach. B3RP3 mRNAs, which also consist of several different transcripts, have a more limited tissue distribution. The highest levels occur in heart, and in gastrointestinal sections the highest levels are in the forestomach. Band 3 mRNAs were observed in many tissues but high levels of expression occurred only in spleen and kidney. Five Band 3 transcripts, ranging in size from 3.6 to 4.9 kilobases, were detected, including three that are expressed in heart.