Effect of probiotic, prebiotic, and synbiotic on the gut microbiota of autistic children using an in vitro gut microbiome model
Effect of probiotic, prebiotic, and synbiotic on the gut microbiota of autistic children using an in vitro gut microbiome model
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DOI：
10.1016/j.foodres.2021.110657
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发表时间：
2021-09-03
影响因子：
8.1
通讯作者：
Sivieri, Katia
中科院分区：
文献类型：
--
作者：
Duque, Ana Luiza Rocha Faria;Demarqui, Fernanda Manaia;Sivieri, Katia
肠道微生物群组成的不平衡发生在患有自闭症谱系障碍（ASD）的个体中。益生菌，益生元和合生元的管理正在成为调节肠道微生物群和改善ASD相关症状的潜在和有前途的策略。我们首先研究了益生菌Limosilaclactin（L.）reuteri和双歧杆菌（B.）在模拟胃肠道条件下，单独、混合和与低聚半乳糖（GOS）组合的longum。接下来，我们评估了益生菌（L。reuteri + B。longum）、益生元（GOS）和合生元（L. reuteri + B。longum + GOS）对患有ASD的儿童的肠道微生物群组成和代谢的影响。L. reuteri，B. longum和GOS显示胃肠道阻力升高。益生菌，益生元和合生素治疗导致ASD儿童肠道微生物群和代谢活动的积极调节。更具体地，益生菌处理增加了乳杆菌属的相对丰度，而益生元处理增加了双歧杆菌属的相对丰度并降低了毛梭菌属的相对丰度。微生物代谢的变化与短链脂肪酸浓度的增加和铵水平的降低有关，特别是在益生元和合生素治疗中。
Imbalances in gut microbiota composition occur in individuals with autism spectrum disorder (ASD). The administration of probiotics, prebiotics, and synbiotics is emerging as a potential and promising strategy for regulating the gut microbiota and improving ASD-related symptoms. We first investigated the survival of the probiotics Limosilactobacillus (L.) reuteri and Bifidobacterium (B.) longum alone, mixed and combined with a galacto-oligosaccharide (GOS) under simulated gastrointestinal conditions. Next, we evaluated the impact of probiotics (L. reuteri + B. longum), prebiotic (GOS), and synbiotic (L. reuteri + B. longum + GOS) on gut microbiota composition and metabolism of children with ASD using an in vitro fermentation model (SHIME (R)). The combination of L. reuteri, B. longum, and GOS showed elevated gastrointestinal resistance. The probiotic, prebiotic, and synbiotic treatments resulted in a positive modulation of the gut microbiota and metabolic activity of children with ASD. More specifically, the probiotic treatment increased the relative abundance of Lactobacillus, while the prebiotic treatment increased the relative abundance of Bifidobacterium and decreased the relative abundance of Lachnoclostridium. Changes in microbial metabolism were associated with increased short-chain fatty acid concentrations and reduced ammonium levels, particularly in the prebiotic and synbiotic treatments.