INDUCTION OF A GROWTH-PHASE-DEPENDENT PROMOTER TRIGGERS TRANSCRIPTION OF BOLA, AN ESCHERICHIA-COLI MORPHOGEN
INDUCTION OF A GROWTH-PHASE-DEPENDENT PROMOTER TRIGGERS TRANSCRIPTION OF BOLA, AN ESCHERICHIA-COLI MORPHOGEN
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DOI：
10.1002/j.1460-2075.1989.tb08573.x
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发表时间：
1989-12-01
期刊：
影响因子：
11.4
通讯作者：
KUSHNER, SR
中科院分区：
文献类型：
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作者：
ALDEA, M;GARRIDO, T;KUSHNER, SR
bolA基因，这是参与大肠杆菌的形态发生途径，进行了测序，并确定了两个潜在的启动子。从启动子P1的表达，近端的bolA结构基因的过渡期间的生长的稳定期特异性诱导。该启动子含有一个非usal-10区（CGGCTAGTA），它定义了一类新的大肠杆菌。在对数生长停止期间，大量蛋白质合成速率急剧增加所必需的大肠杆菌启动子。这一结论通过鉴定另外两个E. coli启动子和一个质粒启动子，它们也在向生长稳定期过渡期间被诱导。分析期间产生的蛋白质的生长在一个bolA无效突变体的指数和稳定期的建议可能的作用，为bolA蛋白在诱导青霉素结合蛋白6（PBP 6）的表达在过渡到稳定期。支持这一假设的是一个假定的DNA结合结构域内的bolA编码序列的存在。
The bolA gene, which is involved in the morphogenetic pathways of Escherichia coli, was sequenced and two potential promoters were identified. Expression from promoter P1, proximal to the bolA structural gene is specifically induced during the transition to the stationary phase of growth. This promoter contains an unusal -10 region (CGGCTAGTA), which defines a new class of E. coli promoters necessary for the dramatic increase in the rate of synthesis of a large set of proteins during the cessation of logarithmic growth. This conclusion was confirmed by identifying two additional E. coli promoters and one plasmid promoter, which also were induced during the transition to the stationary phase of growth. Analysis of proteins produced during the exponential and stationary phases of growth in a bolA null mutant suggest a possible role for the BolA protein in the induction of the expression of penicillin-binding protein 6 (PBP6) in the transition to the stationary phase. Supporting this hypothesis is the presence of a putative DNA-binding domain within the bolA coding sequence.