An integrated proteomic and metabolomic study on the gender-specific responses of mussels Mytilus galloprovincialis to tetrabromobisphenol A (TBBPA)
An integrated proteomic and metabolomic study on the gender-specific responses of mussels Mytilus galloprovincialis to tetrabromobisphenol A (TBBPA)
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贻贝对四溴双酚 A (TBBPA) 性别特异性反应的蛋白质组学和代谢组学综合研究
DOI：
10.1016/j.chemosphere.2015.08.052
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发表时间：
2016-02-01
期刊：
影响因子：
8.8
通讯作者：
Wu, Huifeng
中科院分区：
文献类型：
--
作者：
Ji, Chenglong;Li, Fei;Wu, Huifeng
四溴双酚A（TBBPA）是我国沿着溴系阻燃剂（BFR）的主要来源，其毒性多样，备受关注。在这项研究中，我们专注于性别特异性反应TBBPA贻贝galloprovincialis使用集成的蛋白质组学和代谢组学方法。TBBPA（10 μ g L ~（-1））染毒1个月后，染毒组贻贝鳃中共有9种代谢产物和67种蛋白质发生变化。暴露组雌蚌鳃中代谢物的显著变化反映了能量代谢和渗透调节的紊乱，而雄蚌鳃中仅发现了与渗透调节有关的代谢物的变化。基于iTRAQ的蛋白质组学分析显示了来自溶剂对照组的雄性和雌性贻贝鳃之间的生物学差异。雄性贻贝鳃中与初级代谢、能量代谢和防御机制相关的蛋白质含量较高，说明雄性贻贝具有较强的抗应激能力。进一步分析表明，TBBPA暴露对雌、雄贻贝的生产发育、物质能量代谢、信号转导、基因表达、防御机制和细胞凋亡等多个生物学过程产生了不同的影响，其作用机制也不尽相同。特别是雄性贻贝对TBBPA的反应蛋白的耐受极限高于雌性个体，这与溶剂对照组雌雄贻贝鳃的生物学差异相一致。这一工作表明，在生态毒理学中应考虑性别差异。(C)2015爱思唯尔有限公司版权所有。
Tetrabromobisphenol A (TBBPA), accounting for the largest production of brominated flame-retardants (BFRs) along the Laizhou Bay in China, is of great concern due to its diverse toxicities. In this study, we focused on the gender-specific responses of TBBPA in mussel Mytilus galloprovincialis using an integrated proteomic and metabolomic approach. After exposure of TBBPA (10 mu g L-1) for one month, a total of 9 metabolites and 67 proteins were altered in mussel gills from exposed group. The significant changes of metabolites in female mussel gills from exposed group exhibited the disturbances in energy metabolism and osmotic regulation, while in male samples only be found the variation of metabolites related to osmotic regulation. iTRAQ-based proteomic analysis showed biological differences between male and female mussel gills from solvent control group. The higher levels of proteins related to primary and energy metabolism and defense mechanisms in male mussel gills meant a greater anti-stress capability of male mussels. Further analysis revealed that TBBPA exposure affected multiple biological processes consisting of production and development, material and energy metabolism, signal transduction, gene expression, defense mechanisms and apoptosis in both male and female mussels with different mechanisms. Specially, the responsive proteins of TBBPA in male mussels signified higher tolerance limits than those in female individuals, which was consistent with the biological differences between male and female mussel gills from solvent control group. This work suggested that the gender differences should be considered in ecotoxicology. (C) 2015 Elsevier Ltd. All rights reserved.