Seeding Protein Crystallization with Cross-Linked Protein Crystals
Seeding Protein Crystallization with Cross-Linked Protein Crystals
复制标题
用交联蛋白质晶体接种蛋白质结晶
DOI：
10.1021/acs.cgd.7b01536
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发表时间：
2018-01
影响因子：
3.8
通讯作者：
Yin Da-Chuan
中科院分区：
文献类型：
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作者：
Yan Er-Kai;Zhao Feng-Zhu;Zhang Chen-Yan;Yang Xue-Zhou;Shi Miao;He Jin;Liu Ya-Li;Liu Yue;Hou Hai;Yin Da-Chuan
蛋白质结晶非常重要，因为蛋白质晶体具有许多不同的重要应用，包括蛋白质的大规模纯化、蛋白质结构的测定、纳米颗粒制备和结晶的理论研究。通常用于有效结晶蛋白质的方法是使用成核剂或种子（蛋白质晶体的小片段），其可以帮助增加蛋白质结晶的可能性。由于种子对蛋白质结晶具有非常积极的作用，种子现已被广泛接受并用于实际蛋白质结晶中。在这里，我们表明，交联蛋白质晶体（CLPC），它保留了晶体结构，但比非交联晶体更稳定，也可以用作一种新型的种子，促进蛋白质结晶。用CLPC接种对蛋白质晶体的再现性和筛选都有影响，并且可以改善蛋白质晶体的光学完整性（定义明确的小面）。
Protein crystallization is of great importance because protein crystals have a number of different important applications, including large-scale purification of proteins, determination of protein structure, nanoparticle preparation, and theoretical studies of crystallization. An approach often used to efficiently crystallize proteins is the use of nucleants or seeds (small fragments of protein crystals) that can help increase the probability of protein crystallization. Due to the very positive effect that seeding has on protein crystallization, seeds are now widely accepted and utilized in practical protein crystallization. Here, we show that cross-linked protein crystals (CLPCs), which retain the crystal structure but are much more stable than non-cross-linked crystals, can also be used as a new type of seed for promoting protein crystallization. Seeding with CLPCs has effects on both the reproducibility and screening of protein crystals and could improve the optical perfection (well-defined facets) of p...