YKT6 is a core constituent of membrane fusion machineries at the Arabidopsis trans-Golgi network
YKT6 is a core constituent of membrane fusion machineries at the Arabidopsis trans-Golgi network
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DOI：
10.1016/j.jmb.2005.04.061
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发表时间：
2005-07-01
影响因子：
5.6
通讯作者：
Bassham, DC
中科院分区：
文献类型：
--
作者：
Chen, Y;Shin, YK;Bassham, DC
SNARE复合物的形成是胞外和空泡运输途径中膜融合的必要条件。囊泡相关SNARE （v-）与靶膜SNARE （t-）结合形成SNARE复合体，桥接两个膜，可能促进膜融合。拟南芥基因组编码大量可预测的SNARE蛋白，这些蛋白可能主要作为内膜系统中囊泡运输的融合原。SNAREs SYP41， SYP61和VTI12存在于反式高尔基网络中，并被认为在与该细胞器的囊泡融合中共同起作用。在这里，我们使用脂质体融合实验来证明VTI12和SYP41或SYP61中的任何一个都是膜融合所必需的，但不是两者都需要。这表明SYP41和SYP61可能在拟南芥中独立的囊泡融合反应中起作用。此外，我们还鉴定了两个新的功能可互换的成分YKT61和YKT62，它们与多功能酵母SNARE YKT6序列相似。YKT61和YKT62都与SYP41相互作用，对SYP41或SYP61介导的膜融合至关重要。因此，这些结果确定了拟南芥反式高尔基网络中膜融合所需的核心成分。(c) 2005 Elsevier Ltd版权所有。
SNARE complex formation is essential for membrane fusion in exocytotic and vacuolar trafficking pathways. Vesicle-associated (v-) SNARE associates with a target membrane (t-) SNARE to form a SNARE complex bridging two membranes, which may facilitate membrane fusion. The Arabidopsis genome encodes a large number of predicted SNARE proteins that might function primarily as fusogens for vesicle transport in endomembrane systems. The SNAREs SYP41, SYP61 and VTI12 reside in the trans-Golgi network and have been proposed to function together in vesicle fusion with this organelle. Here, we use a liposome fusion assay to demonstrate that VTI12 and either SYP41 or SYP61, but not both, are required for membrane fusion. This indicates that SYP41 and SYP61 are likely to function in independent vesicle fusion reactions in Arabidopsis. In addition, we have identified two new functionally interchangeable components, YKT61 and YKT62, that show sequence similarity to the multifunctional yeast SNARE YKT6. Both YKT61 and YKT62 interact with SYP41 and are essential for membrane fusion mediated by either SYP41 or SYP61. These results therefore define the core constituents required for membrane fusion at the Arabidopsis trans-Golgi network. (c) 2005 Elsevier Ltd. All rights reserved.