[The relationship between intracellular chloride concentration and ischemia reperfusion-induced arrhythmias in myocardial cells].
[The relationship between intracellular chloride concentration and ischemia reperfusion-induced arrhythmias in myocardial cells].
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发表时间：
2002-04
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通讯作者：
Z. Lai
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作者：
Z. Lai
我们研究了模拟缺血对豚鼠心室心肌细胞内 Cl- 浓度 ([Cl-]i) 的影响，以及 [Cl-]i 对灌注大鼠心脏缺血/再灌注诱发的心律失常的可能作用。我们的结果提供了直接证据，表明缺血条件下心室肌中的[Cl-]i 增加，这表明缺血引起的Cl-(-)HCO3-交换器的激活将部分导致[Cl-]i 的升高。应用二苯乙烯衍生物或降低灌注液中的 Cl- 浓度可延缓缺血引起的动作电位、pHi、[Cl-]i 恶化的发生，并抑制缺血/再灌注引起的心律失常的发生。结论强调了细胞内Cl-稳态在心脏生理和心肌缺血/再灌注损伤发病机制中的重要作用。
We investigated the effects of simulated ischemia on intracellular Cl- concentration ([Cl-]i) in guinea pig ventricular myocardial cells and possible role of the [Cl-]i on the ischemia/reperfusion-induced arrhythmias in perfused rat hearts. Our results provided direct evidence that the [Cl-]i in ventricular muscle was increased under ischemic conditions, which suggested that activation of the Cl-(-)HCO3- exchanger by ischemia would partially contribute to the elevation of [Cl-]i. Application of stilbene derivatives or lowering Cl- concentration in perfusion solution delayed the onset of ischemia-induced deterioration in action potentials, pHi, [Cl-]i, and suppressed the incidence of ischemia/reperfusion-induced arrhythmias. The conclusion was made to emphasize the important role of intracellular Cl- homeostasis in cardiac physiology and pathogenesis of myocardial ischemia/reperfusion injury.