Genetic divergence at the synaptophysin (Syp I) locus among Norwegian coastal and north-east Arctic populations of Atlantic cod
Genetic divergence at the synaptophysin (Syp I) locus among Norwegian coastal and north-east Arctic populations of Atlantic cod
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DOI：
10.1006/jfbi.1997.0491
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发表时间：
1997-11-01
影响因子：
2
通讯作者：
Pogson, GH
中科院分区：
文献类型：
--
作者：
Fevolden, SE;Pogson, GH
几个核RFLP基因座已被发现，最近表现出广泛的等位基因频率变化之间的挪威沿海和东北北极地区的大西洋鳕鱼Gadus morhua的人口。这些多态性之一是通过杂交一个匿名的cDNA克隆（GM 798）对基因组DNA用限制性内切酶DraI消化。该cDNA克隆现已被测序并鉴定为突触素（Syp I），一种整合的突触囊泡膜蛋白。构建引物，其扩增Syp I基因的内含子，所述内含子对于Dial位点是多态性的，从而使得可以使用基于PCR的测定来对多态性进行评分。从父母海，沿海地区和峡湾在北方挪威共965个人抽样进行了分析，这种多态性。结果证实，东北北极和沿海鳕鱼之间存在非常显着的差异在Syp I位点。聚类分析显示，沿海和北极人口之间的深刻分裂和层次F-统计表明，约40%的总变化是由于北极和沿海群体之间的差异。等位基因频率的时间稳定性进行了评估，通过比较Syp I等位基因频率之间的样本中的青少年（0组）捕获在特定位置的峡湾在连续几年中，成年人和青少年收集的样本从同一峡湾。在1994年和1995年在马兰根收集的青少年样本在遗传上没有区别，而从Donnesfjord和Ullsfjorden在同一个2年期间采样的青少年表现出显着的差异。成年人和0组收集的个人从同一峡湾被发现是遗传上无法区分的马兰根，但不是在Balsfjorden。除了检测北极和沿海群体之间的巨大差异，Syp I基因座表明，鳕鱼在不同的峡湾和整个北方挪威的人口之间的基因流的居民群体之间存在遗传异质性可能大大低于以前认为的。(C)1997年，不列颠群岛渔业协会。
Several nuclear RFLP loci have been discovered recently that exhibit extensive allele frequency variation among Norwegian coastal and north-east Arctic populations of Atlantic cod Gadus morhua. One of these polymorphisms was detected by hybridizing an anonymous cDNA clone (GM798) against genomic DNA digested with the restriction enzyme DraI. This cDNA clone has now been sequenced and identified as synaptophysin (Syp I), an integral synaptic vesicle membrane protein. Primers were constructed that amplify an intron of the Syp I gene that is polymorphic for the Dial site, thus making it possible to use a PCR-based assay to score the polymorphism. A total of 965 individuals sampled from the Parents Sea, coastal areas and fjords in northern Norway have been analysed for this polymorphism. The results confirm that highly significant differences exist between NE Arctic and coastal cod at the Syp I locus. A cluster analysis revealed a deep split between coastal and Arctic populations and hierarchical F-statistics indicated that about 40% of the total variation was attributable to differences between Arctic and coastal groups. The temporal stability of allele frequencies was assessed by comparing Syp I allele frequencies among samples of juveniles (0 group) captured at specific locations in fjords in consecutive years and among samples of adults and juveniles collected from the same fjord. Samples of juveniles collected in 1994 and 1995 in Malangen were genetically indistinguishable whereas juveniles sampled from Donnesfjord and Ullsfjorden over the same 2-year period exhibited significant differences. Adults and 0-group individuals collected from the same fjord were found to be genetically indistinguishable in Malangen, but not in Balsfjorden. In addition to detecting large differences among Arctic and coastal groups, the Syp I locus suggests that genetic heterogeneity exists among resident populations of cod in different fjords and that gene flow among populations throughout northern Norway may be considerably lower than previously believed. (C) 1997 The Fisheries Society of the British Isles.