Evaluation of different methods for assessment of insulin sensitivity in Gottingen minipigs: introduction of a new, simpler method
Evaluation of different methods for assessment of insulin sensitivity in Gottingen minipigs: introduction of a new, simpler method
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DOI：
10.1152/ajpregu.90851.2008
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发表时间：
2009-10-01
影响因子：
2.8
通讯作者：
Pacini, Giovanni
中科院分区：
文献类型：
--
作者：
Christoffersen, Berit;Ribel, Ulla;Pacini, Giovanni
Christoffersen B、Ribel U、Raun K、Golozoubova V、Pacini G。评估哥廷根小型猪胰岛素敏感性的不同方法的评估：引入一种新的、更简单的方法。 Am J Physiol Regul Integr Comp Physiol 297：R1195-R1201，2009。首次发表于 2009 年 8 月 26 日； doi:10.1152/ajpregu.90851.2008.-在糖尿病研究中使用动物模型需要可靠的测试来评估胰岛素敏感性和 β 细胞功能。小型猪越来越多地用于代谢研究，本研究的目的是比较不同的测试和指标，以评估哥廷根小型猪的胰岛素敏感性和β细胞功能。在饲喂低能量或高能量饮食的小型猪中进行高胰岛素、等血糖钳夹、静脉注射（IVGTT）和口服葡萄糖耐量试验（OGTT）以及改良的胰岛素耐量试验。此外，还评估了 IVGTT 衍生参数的再现性。先前描述的胰岛素敏感性指标[稳态葡萄糖输注速率/葡萄糖浓度/钳夹胰岛素浓度(M/G/I)；口服葡萄糖胰岛素敏感性 (OGIS) 和 OGTT 的 ISIcomp； S-I来自IVGTT的最小模型分析；和空腹值定量胰岛素敏感性检查指数]与改良胰岛素耐量试验（ISIITT）的胰岛素敏感性指数和IVGTT前30分钟衍生的新简单指数（S2）一起计算。通过 IVGTT 和 OGTT 评估 β 细胞功能。 IVGTT 衍生参数的再现性计算为个体内变异系数中值 (CV%)。 M/G/I 仅与 S2 显着相关（P < 0.05，r = 0.54）。 S2 还与 S-I (P < 0.001，r = 0.81)、ISIITT (P < 0.001，r = 0.57) 以及 OGTT、ISIcomp (P < 0.001，r = 0.78) 和 OGIS (p < 0.05，r = 0.48) 的两个指数相关。 OGTT 和 IVGTT 的 β 细胞功能指标之间没有发现相关性。新 S2 指数的中位 CV% 为 13。 总之，新的简单胰岛素敏感性指数 S2 可用于评估猪的胰岛素敏感性。
Christoffersen B, Ribel U, Raun K, Golozoubova V, Pacini G. Evaluation of different methods for assessment of insulin sensitivity in Gottingen minipigs: introduction of a new, simpler method. Am J Physiol Regul Integr Comp Physiol 297: R1195-R1201, 2009. First published August 26, 2009; doi:10.1152/ajpregu.90851.2008.-The use of animal models in diabetes research requires reliable tests for evaluation of insulin sensitivity and beta-cell function. Minipigs are being increasingly used in metabolic research, and the aim of this study was to compare different tests and indexes for evaluation of insulin sensitivity and beta-cell function in Gottingen minipigs. Hyperinsulinemic, isoglycemic clamp, intravenous (IVGTT) and oral glucose tolerance tests (OGTT), and a modified insulin tolerance test were performed in minipigs fed either low-or high-energy diet. Furthermore, the reproducibility of IVGTT-derived parameters was assessed. Previously described insulin sensitivity indexes [steady-state glucose infusion rate/glucose concentration/insulin concentration from clamp (M/G/I); oral glucose insulin sensitivity (OGIS) and ISIcomp from OGTT; S-I from minimal model analysis of IVGTT; and quantitative insulin sensitivity check index from fasting values] were calculated together with an insulin sensitivity index from the modified insulin tolerance test (ISIITT) and a new simple index (S2) derived from the first 30 min of the IVGTT. beta-Cell function was assessed from the IVGTT and the OGTT. Reproducibility of the IVGTT-derived parameters was calculated as median intraindividual coefficient of variation (CV%). M/G/I correlated significantly only with S2 (P < 0.05, r = 0.54). S2 furthermore correlated with S-I (P < 0.001, r = 0.81), ISIITT (P < 0.001, r = 0.57), and the two indexes from OGTT, ISIcomp (P < 0.001, r = 0.78) and OGIS (p < 0.05, r = 0.48). No correlation was found between beta-cell function indexes from OGTT and IVGTT. The median CV% of the new S2 index was 13. In conclusion, the new simple index of insulin sensitivity, S2, was revealed to be useful for evaluation of insulin sensitivity in pigs.