Characterization of patterned self-assembled monolayers and protein arrays generated by the ink-jet method
Characterization of patterned self-assembled monolayers and protein arrays generated by the ink-jet method
复制标题
DOI：
10.1021/la026171u
复制
发表时间：
2003-03-04
期刊：
影响因子：
3.9
通讯作者：
Boland, TJ
中科院分区：
文献类型：
--
作者：
Pardo, L;Wilson, WC;Boland, TJ
商业喷墨打印机使用小的修改，存款烷硫醇上的金基板和几个蛋白质上的二氧化硅支持。由此产生的模式的烷硫醇形成自组装层相比，通过微接触印刷或溶液吸附获得的那些。该方法已被成功地用于创建二元化学梯度和模式的三级功能。蛋白质在基质上形成密集的图案，并且似乎保持其构型，如通过其结合其特异性配体的能力所测量的。同时打印四种不同的蛋白质，允许阳性和阴性对照。这种“按需滴注”的印刷方法是目前化学功能化表面的二元技术的廉价、灵活的替代方案。
Commercial ink-jet printers were used with little modification to deposit alkanethiols onto gold substrata and several proteins onto silica supports. The resulting patterns of alkanethiols form self-assembled layers comparable to those obtained by microcontact printing or solution adsorption. The method has been used successfully to create binary chemical gradients and patterns of tertiary functionality. The proteins form dense patterns on the substrates and seem to maintain their configuration as measured by their ability to bind their specific ligands. Four different proteins were printed simultaneously, allowing for positive and negative controls. This "drop-on-demand" printing method is an inexpensive, flexible alternative to current binary technologies of chemically functionalizing surfaces.