Sequences and Polymorphisms of the Canine Monoamine Transporter Genes SLC6A2, SLC6A3, and SLC6A4 among Five Dog Breeds
Sequences and Polymorphisms of the Canine Monoamine Transporter Genes SLC6A2, SLC6A3, and SLC6A4 among Five Dog Breeds
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DOI：
10.1292/jvms.70.971
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发表时间：
2008-09-01
影响因子：
1.2
通讯作者：
Mori, Yuji
中科院分区：
文献类型：
--
作者：
Arata, Sayaka;Ogata, Niwako;Mori, Yuji
单胺转运蛋白具有调节中枢神经系统突触间隙单胺浓度的作用，是抗抑郁药和精神兴奋剂的靶标。它们包括去甲肾上腺素、多巴胺和血清素的转运蛋白，分别由 SLC6A2、SLC6A3 和 SLC6A4 基因编码。我们对狗的这些基因编码区的全长进行了测序，并在 SLC6A2 中鉴定出四个单核苷酸多态性 (SNP)，在 SLC6A3 中鉴定出四个单核苷酸多态性 (SNP)。一个 SLC6A3 SNP 是非同义的，导致前面的苏氨酸氨基酸替换为丝氨酸。这些多态性的基因型频率在具有不同行为特征的五个品种中存在显着差异，表明新的 SLC6A2 和 SLC6A3 SNP 将为狗的行为遗传学研究提供额外的有用信息。
Monoamine transporters have roles to regulate the monoamine concentrations in synaptic clefts in the central nervous system and are the targets of antidepressants and psychostimulants. They include transporters for norepinephrine, dopamine and serotonin, which are encoded by the SLC6A2, SLC6A3, and SLC6A4 genes, respectivily. We sequenced the full lengths of the coding regions of these genes for dogs and identified four single nucleotide polymorphisms (SNPs) in SLC6A2 and four in SLC6A3. One SLC6A3 SNP was non-synonymous and caused an amino acid substitution front threonine to serine. The genotype frequencies of these polymorphisms differed significantly among five breeds with different behavioral traits, suggesting that novel SLC6A2 and SLC6A3 SNPs would provide additional useful information for behavioral genetic studies in dogs.