Hearing Protection Outcomes of Analog Electrode Arrays Coated with Different Drug-Eluting Polymer Films Implanted into Guinea Pig Cochleae.
Hearing Protection Outcomes of Analog Electrode Arrays Coated with Different Drug-Eluting Polymer Films Implanted into Guinea Pig Cochleae.
复制标题
涂有不同药物洗脱聚合物薄膜的模拟电极阵列植入豚鼠耳蜗的听力保护效果
DOI：
10.2147/dddt.s318117
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Yang J
中科院分区：
文献类型：
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作者：
Huang Y;Yu H;Liang M;Hou S;Chen J;Zhang F;Sun X;Jia H;Yang J
目的探讨不同药物洗脱模拟电极阵列植入豚鼠耳蜗的听力保护效果。方法将60只豚鼠随机分为阴性对照组和5个实验组，分别植入空白（药物载体）、地塞米松（DXM）、阿糖胞苷（Ara-C）、阿糖胞苷+地塞米松（Ara-C + DXM）和烟酰胺腺嘌呤二核苷酸（NAD+）洗脱模拟电极阵列。显微CT用于监督手术过程。对豚鼠听性脑干反应（ABR）阈值进行测定和分析。结果与结论与阴性对照组比较，术后各处理组ABR阈值均明显升高（P <0.001）。其中，空白组（0 vs 90 d：59.70 ± 10.57 vs 64.60 ± 9.47 dB SPL）与NAD+组（0 vs 90 d：59.90 ± 9.87 vs 64.70 ± 8.65 dB SPL）无明显差异。DXM组（0天：58.10 ± 10.73 dB SPL; 90天：51.70 ± 9.07 dB SPL）和Ara-C组（0天：59.00 ± 10.05 dB SPL; 90天：51.60 ± 8.48 dB SPL）的ABR阈值较前两组均显著降低（p <0.001）。然而，Ara-C + DXM组没有显示出进一步的益处（p> 0.05）。此外，阿糖胞苷和（或）地塞米松组耳蜗螺旋神经节神经元的存活率明显高于对照组。
Objective To investigate the hearing protection outcomes of different drug-eluting analog electrode arrays implanted into guinea pig cochleae. Methods Sixty guinea pigs were randomly divided into a negative control group and five experimental groups implanted separately with blank (drug carrier), dexamethasone (DXM), aracytine (Ara-C), Ara-C+DXM, and nicotinamide adenine dinucleotide (NAD+) eluting analog electrode arrays. Micro CT was used to supervise the surgical procedure. Auditory brainstem response (ABR) thresholds of the guinea pigs were measured and analyzed. Results and Conclusions Compared with the negative control, all other groups showed a significant increase in ABR threshold (p<0.001) after surgery. Among them, there was no obvious difference between the blank (0 vs 90 days: 59.70±10.57 vs 64.60±9.47 dB SPL) and the NAD+ group (0 vs 90 days: 59.90±9.87 vs 64.70±8.65 dB SPL). On the other hand, the ABR thresholds in the DXM (0 days: 58.10±10.73 dB SPL; 90 days: 51.70±9.07 dB SPL) and the Ara-C group (0 days: 59.00±10.05 dB SPL; 90 days: 51.60±8.48 dB SPL) decreased significantly compared with the former two groups (p<0.001). However, the Ara-C+DXM group showed no further benefit (p>0.05). In addition, a significantly higher survival rate of spiral ganglion neurons in cochleae was observed in the Ara-C and/or DXM groups.