The biocompatibility of quantum dot probes used for the targeted imaging of hepatocellular carcinoma metastasis
The biocompatibility of quantum dot probes used for the targeted imaging of hepatocellular carcinoma metastasis
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肝细胞癌转移靶向成像量子点探针的生物相容性
DOI：
10.1016/j.biomaterials.2008.07.025
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发表时间：
2008-11-01
期刊：
影响因子：
14
通讯作者：
Li, Yan
中科院分区：
文献类型：
--
作者：
Chen, Liang-Dong;Liu, Jia;Li, Yan
与有机染料相比，半导体量子点具有许多光物理优势，使其成为生物医学应用的良好标记物。用甲胎蛋白(AFP)单抗标记的最大发射波长为590 nm的CdSe/ZnS量子点(QD590)检测人肝癌细胞株HCCLM6细胞质中的AFP。在体内研究中，我们使用QD-AFP-Ab探针通过将人肝癌移植瘤注射到尾静脉来靶向成像在裸鼠体内生长的人肝癌移植瘤。此外，还研究了这些探针的体外细胞毒性、体内急性毒性、血流动力学和组织分布。体外和体内实验结果表明，在我们的肝癌模型系统中，基于QD的探针对分子靶标的超灵敏荧光成像具有良好的稳定性、特异性和生物相容性。(C)2008爱思唯尔有限公司。保留所有权利。
Semiconductor quantum dots (QDs) have several photo-physical advantages over organic dyes making them good markers in biomedical application. We used CdSe/ZnS QDs with maximum emission wavelength of 590 nm (QD590) linked to alpha-fetoprotein (AFP) monoclonal antibody (Ab) to detect AFP in cytoplasm of human hepatocellular carcinoma (HCC) cell line HCCLM6. For the in vivo studies, we used QD-AFP-Ab probes for targeted imaging of human HCC xenograft growing in nude mice by injecting them into the tail vein. In addition, the cytotoxicity in vitro, the acute toxicity in vivo, the hemodynamics and tissue distribution of these probes were also investigated. The results in vitro and in vivo indicate that our QD-based probes have good stability, specificity and biocompatibility for ultrasensitive fluorescence imaging of molecular targets in our liver cancer model system. (C) 2008 Elsevier Ltd. All rights reserved.