Effects of dietary restriction on mortality and age‐related phenotypes in the short‐lived fish Nothobranchius furzeri
Effects of dietary restriction on mortality and age‐related phenotypes in the short‐lived fish Nothobranchius furzeri
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DOI：
10.1111/j.1474-9726.2009.00455.x
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发表时间：
2009-04
期刊：
影响因子：
7.8
通讯作者：
E. Terzibasi;C. Lefrançois;P. Domenici;N. Hartmann;Michael Graf;A. Cellerino
中科院分区：
文献类型：
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作者：
E. Terzibasi;C. Lefrançois;P. Domenici;N. Hartmann;Michael Graf;A. Cellerino
短寿命的一年生鱼类Nothobranchius furzeri显示出极短的圈养寿命和年龄标记的加速表达，使其成为研究实验操作对寿命和年龄相关病理学影响的有趣模型系统。在这里，我们测试了饮食限制（DR）对N的死亡率和年龄相关标志物的影响。furzeri。通过每隔一天喂食诱导DR，并在近交实验室品系和寿命较长的野生衍生品系中进行处理。在近交系实验室中，DR降低了与年龄相关的风险并延长了最大寿命。在野生衍生系中，DR诱导早期死亡，没有降低一般年龄相关风险，并导致最大寿命的小但显著延长。年龄依赖性死亡率分析显示，DR降低了人口老龄化率，但增加了野生型菌株的基线死亡率。在近交系和野生系中，DR阻止了肝脏中年龄标志物脂褐素和大脑中Fluoro-Jade B（神经变性）的表达。DR还提高了基于条件反射的学习测试中的表现（穿梭箱中的主动回避）。最后，DR诱导了脑中胶质细胞酸性蛋白的反常上调。
The short‐lived annual fish Nothobranchius furzeri shows extremely short captive life span and accelerated expression of age markers, making it an interesting model system to investigate the effects of experimental manipulations on longevity and age‐related pathologies. Here, we tested the effects of dietary restriction (DR) on mortality and age‐related markers in N. furzeri. DR was induced by every other day feeding and the treatment was performed both in an inbred laboratory line and a longer‐lived wild‐derived line. In the inbred laboratory line, DR reduced age‐related risk and prolonged maximum life span. In the wild‐derived line, DR induced early mortality, did not reduce general age‐related risk and caused a small but significant extension of maximum life span. Analysis of age‐dependent mortality revealed that DR reduced demographic rate of aging, but increased baseline mortality in the wild‐derived strain. In both inbred‐ and wild‐derived lines, DR prevented the expression of the age markers lipofuscin in the liver and Fluoro‐Jade B (neurodegeneration) in the brain. DR also improved performance in a learning test based on conditioning (active avoidance in a shuttle box). Finally, DR induced a paradoxical up‐regulation of glial fibrillary acidic protein in the brain.