Efficient stem cell labeling for MRI studies
Efficient stem cell labeling for MRI studies
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DOI：
10.1002/cmmi.229
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发表时间：
2008-01-01
影响因子：
--
通讯作者：
Hoehn, M.
中科院分区：
文献类型：
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作者：
Kuestermann, E.;Himmelreich, U.;Hoehn, M.
氧化铁粒子特别适合于细胞跟踪实验，因为与其他顺磁性核相比，它们具有非凡的摩尔弛豫性。我们比较了不同氧化铁颗粒（Sinerem、Endorem和磁性微球）标记胚胎干细胞（D3细胞系）的适用性。除了可检测阈值外，还特别注意了标签程序的长期稳定性评估（长达4周）以及对细胞活力的毒性和其他不利影响。使用神经祖细胞（C17.2）和树突状细胞进行比较研究。本研究表明，标记效率和稳定性与使用中的氧化铁颗粒和细胞系密切相关。版权所有(c) 2008约翰威利父子有限公司
Iron oxide particles are especially suited for cell tracking experiments due to their extraordinarily molar relaxivity as compared with other paramagnetic nuclei. We have compared different iron oxide particles (Sinerem, Endorem and magnetic microspheres) for their suitability to label embryonic stem cells (D3 cell line). In addition to detectability thresholds, particular attention has been paid to the evaluation of long-term stability of the labelling procedure (up to 4 weeks) as well as to toxic and other adverse effects on cell viability. Comparative studies were performed using neural progenitor cells (C17.2) and dendritic cells. The present study indicates strong dependence of the label efficiency and stability on the iron oxide particles and cell lines in use. Copyright (c) 2008 John Wiley & Sons, Ltd.