DEVELOPMENT OF TRYPANOSOMA-CRUZI IN TRIATOMA-INFESTANS - INFLUENCE OF TEMPERATURE AND BLOOD CONSUMPTION
DEVELOPMENT OF TRYPANOSOMA-CRUZI IN TRIATOMA-INFESTANS - INFLUENCE OF TEMPERATURE AND BLOOD CONSUMPTION
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DOI：
10.2307/3283997
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发表时间：
1995-02-01
影响因子：
1.3
通讯作者：
CATALA, S
中科院分区：
文献类型：
--
作者：
ASIN, S;CATALA, S
这项工作有两个目标。首先是利用模拟自然传播过程的实验设计，量化克氏锥虫在两种不同温度下的感染三角虫体内的发展，其次是了解媒介血液消耗率如何改变寄生虫的发展。用x - 1型克氏锥虫（约10(4)只/ml血）感染了203只第五期的感染T. infestans若虫，并将其维持在20和28℃，每天提供机会以未感染的实验小鼠为食。在感染后24小时至第55天，计数直肠和粪便中超环锥乳鞭毛虫总数。在两种温度下的感染餐后第一天开始增重。在两种温度下，这一寄生阶段在病媒体内形成了相似的种群密度。与28℃相比，20℃、32、24 d时，直肠和粪便中出现了锥乳线虫；然而，一旦它们具有传染性，昆虫就会发展出类似的粪便亚循环形式的种群密度。28℃时，血消耗与直肠和直肠锥马鞭毛虫的发育有关，但与粪便中锥马鞭毛虫的数量无关。至少消耗120毫克和180毫克的新鲜血液，确保所有的虫子都发育成粗乳鞭毛虫和粗乳鞭毛虫。尽管在20℃时产生亚循环形式的延迟，但昆虫在两种温度下的感染能力相似。
This work had 2 objectives. The first was to quantify Trypanosoma cruzi development within Triatoma infestans maintained at 2 different temperatures, using an experimental design that simulated the natural transmission process and, second, to learn how the vector blood consumption rate modifies the parasite's development. Two hundred and three, fifth-stage nymphs of T. infestans were infected with the X-l strain of T. cruzi (about 10(4) trypanosomes/ml blood), maintained at 20 and 28 C, and daily offered the opportunity to feed on uninfected laboratory mice. From 24 hr to the 55th day after the infective meal, the total number of epimastigotes and rectal and fecal metacyclic trypomastigotes were counted. Epimastigote multiplication began on the first day after the infective meal at both temperatures. This parasitic stage developed similar population densities within the vector under both temperature regimes. Trypomastigotes appeared in the rectum and feces at 20 C, 32 and 24 days later, respectively, than at 28 C; however, once they became infective, insects developed similar population densities of fecal metacyclic forms. Blood consumption was related to epimastigote and rectal trypomastigote development at 28 C, but not to the number of trypomastigotes in the feces. A minimum of 120 and 180 mg of fresh blood consumed assured that all bugs developed epimastigotes and trypomastigotes. In spite of the delay in producing metacyclic forms at 20 C, the insect's infective capacity was similar at both temperatures.