Fluorophotometric Analysis of the Ocular Surface Glycocalyx in Soft Contact Lens Wearers
Fluorophotometric Analysis of the Ocular Surface Glycocalyx in Soft Contact Lens Wearers
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DOI：
10.3109/02713683.2014.999948
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发表时间：
2016-01-02
影响因子：
2
通讯作者：
Tsubota, Kazuo
中科院分区：
文献类型：
--
作者：
Fukui, Masaki;Yamada, Masakazu;Tsubota, Kazuo
目的：角膜接触透镜配戴者泪膜不稳定，泪膜破裂时间（BUT）缩短，这与配戴者的不适和干燥有关。糖萼被认为是维持眼表湿润性和润滑性的关键。我们评估了眼表糖萼在软接触透镜（SCL）配戴者使用荧光素标记的麦胚凝集素（F-WGA）作为标记，以证明眼表糖复合物在vivo.Methods：20经验丰富的SCL配戴者和20名健康志愿者，没有CL配戴史（对照组）参加了这项研究。在将5% F-WGA溶液应用于研究个体的眼睛后，通过荧光光度法测量其各自中央角膜中的荧光强度。结果：SCL组角膜表面F-WGA荧光强度为418.5 ± 103.3，显著低于对照组（825.0 ± 179.8; P < 0.01），SCL组角膜表面F-WGA荧光强度为418.5 ± 103.3，显著低于对照组（825.0 ± 179.8; P < 0.01）。
Purpose: Unstable tear film characterized by shorting of tear break-up time (BUT) is associated with discomfort and dryness in contact lens wearers. The glycocalyx is thought to be crucial in maintaining the wettability and lubrication of the ocular surface. We evaluated the ocular surface glycocalyx in soft contact lens (SCL) wearers using a fluorescein-labeled wheat germ agglutinin (F-WGA) as a marker to demonstrate the ocular surface glycoconjugates in vivo.Methods: Twenty experienced SCL wearers and 20 healthy volunteers with no history of CL wearing (controls) were enrolled in the study. After applying a 5% F-WGA solution to the eyes of study individuals, fluorescent intensities in their respective central corneas were measured by fluorophotometry. The relationship between F-WGA intensity in the corneal surface and clinical parameters associated with contact lens wear were analyzed.Results: F-WGA fluorescence intensity in the SCL group was 418.5103.3, which was significantly lower than that of the controls (825.0 +/- 179.8; p